Rabbit AXL Polyclonal Antibody | anti-AXL antibody

AXL (AXL Oncogene, AXL Receptor Tyrosine Kinase, AXL Transforming Gene, JTK11, UFO) (FITC)

Cat. Num. AAA6407953

• Gene Names: AXL; UFO; JTK11
• Reactivity: Human, Mouse, Rat
• Applications: Immunoprecipitation, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: AXL; Polyclonal Antibody; AXL (AXL Oncogene; AXL Receptor Tyrosine Kinase; AXL Transforming Gene; JTK11; UFO) (FITC); anti-AXL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes AXL. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-AXL antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 1:20-1:50; WB: 1:500-1:1000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human AXL.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of HepG2 cell line, using AXL antibody.)

Product Categories/Family for anti-AXL antibody

Antibodies; Receptors; Tyrosine Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 21536468
• NCBI GeneID: 558
• NCBI Accession #: NP_001690.2
• NCBI GenBank Nucleotide #: NM_001699.4
• UniProt Accession #: P30530; Q8N5L2; Q9UD27
• Molecular Weight: 99kDa
• NCBI Official Full Name: tyrosine-protein kinase receptor UFO isoform 2
• NCBI Official Synonym Full Names: AXL receptor tyrosine kinase
• NCBI Official Symbol: AXL
• NCBI Official Synonym Symbols: UFO; JTK11
• NCBI Protein Information: tyrosine-protein kinase receptor UFO; AXL oncogene; oncogene AXL; AXL transforming sequence/gene
• UniProt Protein Name: Tyrosine-protein kinase receptor UFO
• Protein Family: Tyrosine-protein kinase receptor
• UniProt Gene Name: AXL
• UniProt Synonym Gene Names: UFO
• UniProt Entry Name: UFO_HUMAN

NCBI Description

The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

AXL: a receptor tyrosine kinase that may function as a signal transducer between specific cell types of mesodermal origin. Interacts with SKP1. Overexpression in tissue culture causes oncogenic transformation. Overexpressed in several cancers including thyroid, ovarian, gastric, ER+ breast cancer and acute myeloid leukemia, where it is associated with poor prognosis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Oncoprotein; Protein kinase, TK; TK group; Axl family

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; phosphatidylserine binding; protein heterodimerization activity; protein-tyrosine kinase activity; phosphoinositide 3-kinase binding; transmembrane receptor protein tyrosine kinase activity; ATP binding; myosin heavy chain binding

Biological Process: forebrain cell migration; negative regulation of lymphocyte activation; platelet activation; organ regeneration; peptidyl-tyrosine phosphorylation; cell maturation; neuron migration; vagina development; negative regulation of tumor necrosis factor production; signal transduction; phagocytosis; positive regulation of natural killer cell differentiation; erythrocyte homeostasis; positive regulation of protein kinase B signaling cascade; ovulation cycle; natural killer cell differentiation; negative regulation of interferon-gamma production; protein kinase B signaling cascade; cellular response to extracellular stimulus; innate immune response; blood vessel remodeling; spermatogenesis; negative regulation of neuron apoptosis; positive regulation of cytokine and chemokine mediated signaling pathway; inflammatory response; vascular endothelial growth factor receptor signaling pathway; apoptotic cell clearance; secretion by cell