Rabbit anti-Mouse Axin 1 Polyclonal Antibody | anti-AXIN1 antibody

Axin 1 Antibody: HRP

Cat. Num. AAA8000422

• Gene Names: AXIN1; AXIN; PPP1R49
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Peptide Affinity Purified
• Synonyms: Axin 1; Polyclonal Antibody; Axin 1 Antibody: HRP; Rabbit Anti-Human Axin 1 Polyclonal; AXIN1 Antibody; hAxin Antibody; Axin-1 Antibody; Axis inhibition protein 1 Antibody; AXIN Antibody; Escherichia coli O119 Antibody; anti-AXIN1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: Detects 100 kDa.
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 862

• Applicable Applications for anti-AXIN1 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Target: Axin 1
• Immunogen: Synthetic peptide of Human Axin 1 (200-300 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Immunogen Species: Human
• Cellular Localization: Cytoplasm; Nucleus; Membrane; Cell Membrane
• Conjugate: HRP
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Tissue Specificity: Ubiquitously expressed.
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Mouse kidney lysate showing detection of ~95.6 kDa Axin 1 protein using Rabbit Anti-Axin 1 Polyclonal Antibody (SPC-721). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse kidney lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Axin 1 Polyclonal Antibody (SPC-721) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~95.6 kDa.)

Product Categories/Family for anti-AXIN1 antibody

Antibodies; Cancer; Cell Cycle; Cell Cycle Inhibitors; Cancer; Tumor Biomarkers; Stem Cells; Signaling Pathways; Wnt/Beta Catenin Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 27501450
• NCBI GeneID: 8312
• NCBI Accession #: NP_003493.1
• NCBI GenBank Nucleotide #: NM_003502.3
• UniProt Accession #: O15169; Q4TT26; Q4TT27; Q86YA7; Q8WVW6; Q96S28
• Molecular Weight: 91,653 Da
• NCBI Official Full Name: axin-1 isoform a
• NCBI Official Synonym Full Names: axin 1
• NCBI Official Symbol: AXIN1
• NCBI Official Synonym Symbols: AXIN; PPP1R49
• NCBI Protein Information: axin-1
• UniProt Protein Name: Axin-1
• Protein Family: Axin
• UniProt Gene Name: AXIN1
• UniProt Synonym Gene Names: AXIN; hAxin

NCBI Description

This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (PubMed:12192039, PubMed:27098453). Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway (PubMed:12192039). In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B (PubMed:12192039). Likely to function as a tumor suppressor. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (PubMed:16601693). Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development (PubMed:17210684). Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation (PubMed:17210684).