Testing Data
(HeLa nuclear extract cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS610712 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrows indicate AUF1 isoforms.)
Rabbit anti-Human AUF1 Polyclonal Antibody | anti-AUF1 antibody
AUF1 (A+U Rich RNA Binding Factor) (MaxLight 490)
WB: 0.01-1ug/ml Detects AUF1 in RIPA lysates from HeLa nuclear extract and A431 cells. HeLa nuclear extract cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with (0.1ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Testing Data
(HeLa nuclear extract cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS610712 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrows indicate AUF1 isoforms.)
Testing Data
(HeLa nuclear extract cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS610712 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrows indicate AUF1 isoforms.)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008]
Uniprot Description
hnRNP D0: a ubiquitously expressed heterogeneous nuclear ribonucleoprotein (hnRNP). Implicated in the regulation of mRNA stability. Has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. Four alternatively spliced transcript variants have been described.
Protein type: RNA splicing; RNA-binding
Chromosomal Location of Human Ortholog: 4q21
Cellular Component: nucleoplasm; ribonucleoprotein complex; nucleus; cytosol
Molecular Function: protein binding; RNA binding; telomeric DNA binding; nucleotide binding
Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; positive regulation of translation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; RNA splicing; RNA catabolic process; gene expression; regulation of mRNA stability; regulation of circadian rhythm
Research Articles on AUF1
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Product Notes
The AUF1 hnrnpd (Catalog #AAA6464503) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AUF1 (A+U Rich RNA Binding Factor) (MaxLight 490) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AUF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. WB: 0.01-1ug/ml Detects AUF1 in RIPA lysates from HeLa nuclear extract and A431 cells. HeLa nuclear extract cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with (0.1ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Researchers should empirically determine the suitability of the AUF1 hnrnpd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AUF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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