Western Blot (WB)
(Western Blotting Analysis: Representative lot data. UV irradiated HeLa cell lysates (Lanes 1-3) or UV irradiated COS1 (Lanes 4-6) lysates were probed with Anti-phospho-ATR (Ser428) (1:200 dilution). Lane 1 and 4: Antibody +No peptide Lane 2 and 5: Antibody +Non-phospho peptide Lane 3 and 6: Antibody +Phospho peptide Proteins were visualized using a Donkey Anti-Rabbit conjugated to HRP and a chemiluminescence detection system. Arrow indicates ATR phosphorylated at (Ser428) (~260kD).)
Rabbit ATR Polyclonal Antibody | anti-ATR antibody
ATR, Phosphorylated (Ser428) (Serine/Threonine-protein Kinase ATR, Ataxia Telangiectasia and Rad3-related Protein, FRAP-related Protein 1, FRP1) (MaxLight 650)
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. UV irradiated HeLa cell lysates (Lanes 1-3) or UV irradiated COS1 (Lanes 4-6) lysates were probed with Anti-phospho-ATR (Ser428) (1:200 dilution). Lane 1 and 4: Antibody +No peptide Lane 2 and 5: Antibody +Non-phospho peptide Lane 3 and 6: Antibody +Phospho peptide Proteins were visualized using a Donkey Anti-Rabbit conjugated to HRP and a chemiluminescence detection system. Arrow indicates ATR phosphorylated at (Ser428) (~260kD).)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. UV irradiated HeLa cell lysates (Lanes 1-3) or UV irradiated COS1 (Lanes 4-6) lysates were probed with Anti-phospho-ATR (Ser428) (1:200 dilution). Lane 1 and 4: Antibody +No peptide Lane 2 and 5: Antibody +Non-phospho peptide Lane 3 and 6: Antibody +Phospho peptide Proteins were visualized using a Donkey Anti-Rabbit conjugated to HRP and a chemiluminescence detection system. Arrow indicates ATR phosphorylated at (Ser428) (~260kD).)
NCBI and Uniprot Product Information
Uniprot Description
ATR: a ser/thr kinase of the PIKK family most closely related to ATM. Activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]Q. Phosphorylates BRCA1, Chk1, MCM2, RAD17, RPA2, SMC1 and p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
Protein type: Kinase, lipid; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Membrane protein, integral; Protein kinase, atypical; Kinase, protein; ATYPICAL group; PIKK family; ATR subfamily
Chromosomal Location of Human Ortholog: 3q23
Cellular Component: XY body; nucleoplasm; Golgi apparatus; PML body; chromosome
Molecular Function: protein dimerization activity; protein serine/threonine kinase activity; protein binding; DNA binding; MutLalpha complex binding; MutSalpha complex binding; ATP binding; protein kinase activity
Biological Process: response to drug; peptidyl-serine phosphorylation; negative regulation of DNA replication; multicellular organismal development; protein amino acid autophosphorylation; regulation of protein binding; DNA damage checkpoint; positive regulation of DNA damage response, signal transduction by p53 class mediator; DNA replication; cell cycle; DNA repair; response to DNA damage stimulus; double-strand break repair via homologous recombination
Disease: Seckel Syndrome 1; Cutaneous Telangiectasia And Cancer Syndrome, Familial
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Product Notes
The ATR atr (Catalog #AAA6444575) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATR, Phosphorylated (Ser428) (Serine/Threonine-protein Kinase ATR, Ataxia Telangiectasia and Rad3-related Protein, FRAP-related Protein 1, FRP1) (MaxLight 650) reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's ATR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ATR atr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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