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Western Blot (WB) (Western Blot analysis of extract of various cells, using ATPIF1 antibody.)

Rabbit anti-Human, Mouse ATPIF1 Polyclonal Antibody | anti-ATPIF1 antibody

ATPIF1 Polyclonal Antibody

Gene Names
ATP5IF1; IP; ATPI; ATPIP; ATPIF1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
ATPIF1; Polyclonal Antibody; ATPIF1 Polyclonal Antibody; IP; ATPI; ATPIP; anti-ATPIF1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ATPIF1 polyclonal antibody detects endogenous levels of ATPIF1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
106
Applicable Applications for anti-ATPIF1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:100
Immunogen
Recombinant protein of human ATPIF1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extract of various cells, using ATPIF1 antibody.)

Western Blot (WB) (Western Blot analysis of extract of various cells, using ATPIF1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using ATPIF1 antibody at dilution of 1:100 (x40 lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach using ATPIF1 antibody at dilution of 1:100 (x40 lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using ATPIF1 antibody at dilution of 1:100 (x40 lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver cancer using ATPIF1 antibody at dilution of 1:100 (x40 lens).)
Related Product Information for anti-ATPIF1 antibody
This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 12kDa
NCBI Official Full Name
ATPase inhibitor, mitochondrial
NCBI Official Synonym Full Names
ATP synthase inhibitory factor subunit 1
NCBI Official Symbol
ATP5IF1
NCBI Official Synonym Symbols
IP; ATPI; ATPIP; ATPIF1
NCBI Protein Information
ATPase inhibitor, mitochondrial
UniProt Protein Name
ATPase inhibitor, mitochondrial
Protein Family
UniProt Gene Name
ATPIF1
UniProt Synonym Gene Names
ATPI; IF(1); IF1
UniProt Entry Name
ATIF1_HUMAN

NCBI Description

This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ATPIF1: Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Homodimer at a pH value below 6.5, inactive homotetramer above. Belongs to the ATPase inhibitor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Mitochondrial

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: cell surface; mitochondrion; mitochondrial proton-transporting ATP synthase complex

Molecular Function: calmodulin binding; enzyme inhibitor activity; angiostatin binding; protein homodimerization activity; ATPase inhibitor activity; ATPase binding

Biological Process: generation of precursor metabolites and energy; regulation of gene expression; negative regulation of hydrolase activity; negative regulation of endothelial cell proliferation; erythrocyte differentiation; mitochondrial depolarization; angiogenesis; negative regulation of nucleotide metabolic process; protein homooligomerization; heme biosynthetic process; protein homotetramerization

Research Articles on ATPIF1

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Product Notes

The ATPIF1 atpif1 (Catalog #AAA3008644) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATPIF1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATPIF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:100. Researchers should empirically determine the suitability of the ATPIF1 atpif1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATPIF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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