Western Blot (WB)
(ATPGD1 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ATPGD1 antibody detected the ATPGD1 protein (arrow).)
Rabbit anti-Human, Mouse ATPGD1 Polyclonal Antibody | anti-ATPGD1 antibody
ATPGD1, NT (CARNS1, ATPGD1, KIAA1394, Carnosine synthase 1, ATP-grasp domain-containing protein 1) (FITC)
Gene Names
CARNS1; ATPGD1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ATPGD1; Polyclonal Antibody; NT (CARNS1; KIAA1394; Carnosine synthase 1; ATP-grasp domain-containing protein 1) (FITC); CARNS1; ATP-grasp domain-containing protein 1; anti-ATPGD1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-ATPGD1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ATPGD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-276 amino acids from the N-terminal region of human ATPGD1.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(ATPGD1 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ATPGD1 antibody detected the ATPGD1 protein (arrow).)
Western Blot (WB)
(ATPGD1 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ATPGD1 antibody detected the ATPGD1 protein (arrow).)
Immunohistochemistry (IHC)
(ATPGD1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATPGD1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ATPGD1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATPGD1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-ATPGD1 antibody
CARNS1 (EC 6.3.2.11), a member of the ATP-grasp family of ATPases, catalyzes the formation of carnosine (beta-alanyl-L-histidine) and homocarnosine (gamma-aminobutyryl-L-histidine), which are found mainly in skeletal muscle and the central nervous system, respectively (Drozak et al., 2010 [PubMed 20097752]).
Product Categories/Family for anti-ATPGD1 antibody
References
Drozak, J., et al. J. Biol. Chem. 285(13):9346-9356(2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
101,320 Da
NCBI Official Full Name
carnosine synthase 1 isoform 2
NCBI Official Synonym Full Names
carnosine synthase 1
NCBI Official Symbol
CARNS1
NCBI Official Synonym Symbols
ATPGD1
NCBI Protein Information
carnosine synthase 1
UniProt Protein Name
Carnosine synthase 1
UniProt Gene Name
CARNS1
UniProt Synonym Gene Names
ATPGD1; KIAA1394
UniProt Entry Name
CRNS1_HUMAN
NCBI Description
CARNS1 (EC 6.3.2.11), a member of the ATP-grasp family of ATPases, catalyzes the formation of carnosine (beta-alanyl-L-histidine) and homocarnosine (gamma-aminobutyryl-L-histidine), which are found mainly in skeletal muscle and the central nervous system, respectively (Drozak et al., 2010 [PubMed 20097752]).[supplied by OMIM, Apr 2010]
Uniprot Description
ATPGD1: Catalyzes the synthesis of carnosine and homocarnosine. Carnosine is synthesized more efficiently than homocarnosine. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.11
Chromosomal Location of Human Ortholog: 11q13.2
Molecular Function: carnosine synthase activity; metal ion binding; ATPase activity; ATP binding
Research Articles on ATPGD1
Similar Products
Product Notes
The ATPGD1 carns1 (Catalog #AAA6276769) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATPGD1, NT (CARNS1, ATPGD1, KIAA1394, Carnosine synthase 1, ATP-grasp domain-containing protein 1) (FITC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATPGD1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-100 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ATPGD1 carns1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATPGD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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