ATPase, Ca, Autoinhibitory Domain Polyclonal Antibody

Anti - ATPase, Ca, Autoinhibitory Domain (c28r2)

Cat. Num. AAA618902

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Serum
• Synonyms: ATPase; Ca; Autoinhibitory Domain; Polyclonal Antibody; Anti - ATPase; Autoinhibitory Domain (c28r2); anti-ATPase; Autoinhibitory Domain antibody

• Host: Host: Rabbit; Source: Human
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The 28 AA human C28R2 sequence is 100% conserved in human Ca+-ATPase isoforms 1, 2, 3b, 96% in 1b, 92% in 4 and 85% in 3a/3b. It is also 100% conserved in mouse and rat isoform 2. A high degree of conservation (77-100%) is also seen in Ca+-ATPase isoforms from many other species. Antibody crossreactivity of C28R2 or its exchange inhibitory properties in various species has not been studied. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
• Purity/Purification: Serum
• Form/Format: Serum; Supplied as a liquid, neat serum, 40% glycerol.

• Applicable Applications for anti-ATPase, Ca, Autoinhibitory Domain antibody: ELISA, Western Blot
• Application Notes: Suitable for use in ELISA, Western Blotting. Other applications not tested.; Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.; ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.; Optimal dilutions to be determined by the researcher.
• Immunogen: The 28 AA Peptide sequence (~CT, cytoplasmic) corresponding to human Ca+-ATPases, autoinhibitory domain (1). The peptide was coupled to KLH
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ATPase, Ca, Autoinhibitory Domain antibody

Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2). The N-terminal signal peptide is cleaved off from the mature exchanger protein. NCX contains a highly basic region in the large hydrophilic, intracellular loop called XIP (Exchange inhibitory peptide; RRLLFYKYVYKRYRAGKQRG 20 aa), that inhibits Na-Ca+ exchange in cardiac sarcolemmal vesicles and in other cells. Little or no sequence identity is found between the NCX and the Ca-pump. However, XIP also inhibits the Ca pumps with more or less same efficiency as C28R2 peptide sequence (LRRGQILWFRGLNRIQTQIRVVKAFRSS, 28 aa) corresponding to the autoinhibitory domain of Ca-pump.

Product Categories/Family for anti-ATPase, Ca, Autoinhibitory Domain antibody

Abs to Enzymes

References

• (1). Street VA et al (1998) Nat. Genet. 19, 390; Heim R et al (1992) Eur. J. Biochem. 205, 330; Brandt P et al (1992) Genomics 14, 484; Latif F et al (1993) Cancer Res. 53, 861; Keeton TP et al (1995) Biochem. J. 306, 779; shull GE et al (1988) J Biol. Chem. 263, 8646; Brown BJ et al (1996) BBA 1283, 10; Hale CC et al (1997) BBRC 236, 113; Xu W et al (1997) Arch. Biochem. Biophys. 341, 273; Blaustein MP and Lederer J (1999) Physiol Rev. 79, 763-854 (review)