Rabbit anti-Human, Mouse ATP7b Polyclonal Antibody

ATP7b (PWD, WC1, WND)

Cat. Num. AAA618416

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by affinity chromatography
• Synonyms: ATP7b; Polyclonal Antibody; ATP7b (PWD; WC1; WND); Anti -ATP7b (PWD; anti-ATP7b antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Recognizes human and mouse ATP7b. This antibody has specifically been tested on mouse brain and liver.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography
• Form/Format: Supplied as a liquid in Tris citrate/phosphate buffer, pH 7.5, 0.1% sodium azide.

• Applicable Applications for anti-ATP7b antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry.; Dilution: Immunohistochemistry (Paraffin): 1:500; Western Blot: 1:1000. Band at ~165kD; Immunocytochemistry: 1:500
• Immunogen: Synthetic peptide made to an internal sequence near the N-terminus of human ATP7b
• Cellular Localization: Golgi apparatus; Predominantly found in trans-Golgi network membrane; multi-pass membrane protein (By similarity). Isoform 2: Cytoplasm. The cleaved form WND/140kD is located in mitochondria.
• Positive Control: HuH7 hepatocarcinoma cell extracts
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ATP7b antibody

ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140kD is found in mitochondria. Mutations in the ATP7b gene can cause Wilson's disease, an inherited disorder causing copper poisoning in the brain and liver.

Product Categories/Family for anti-ATP7b antibody

Antibodies; Abs to Transport Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 11037271
• NCBI Official Full Name: ATP7B

NCBI Description

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq, Jul 2008]

Uniprot Description

ATP7B: Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. Monomer. Interacts with COMMD1/MURR1. Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Vesicle; Transporter; EC 3.6.3.54; Transporter, ion channel; Hydrolase; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: Golgi membrane; mitochondrion; membrane; integral to plasma membrane; perinuclear region of cytoplasm; basolateral plasma membrane; late endosome; cytoplasmic membrane-bound vesicle; trans-Golgi network

Molecular Function: protein binding; copper ion binding; copper-exporting ATPase activity; ATP binding

Biological Process: lactation; cellular copper ion homeostasis; response to copper ion; metabolic process; sequestering of calcium ion; cellular zinc ion homeostasis; copper ion import; copper ion transport; copper ion export; transmembrane transport; intracellular copper ion transport

Disease: Wilson Disease