Rabbit anti-Human, Mouse ATP6V1H Polyclonal Antibody | anti-ATP6V1H antibody

ATP6V1H, CT (ATP6V1H, V-type proton ATPase subunit H, Nef-binding protein 1, Protein VMA13 homolog, V-ATPase 50/57kD subunits, Vacuolar proton pump subunit H, Vacuolar proton pump subunit SFD) (APC)

Cat. Num. AAA6276723

• Gene Names: ATP6V1H; SFD; NBP1; VMA13; CGI-11; MSTP042; SFDbeta; SFDalpha
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATP6V1H; Polyclonal Antibody; CT (ATP6V1H; V-type proton ATPase subunit H; Nef-binding protein 1; Protein VMA13 homolog; V-ATPase 50/57kD subunits; Vacuolar proton pump subunit H; Vacuolar proton pump subunit SFD) (APC); Vacuolar proton pump subunit SFD; anti-ATP6V1H antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ATP6V1H antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ATP6V1H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 407-437 amino acids from the C-terminal region of human ATP6V1H.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATP6V1H Antibody (C-term) in mouse kidney tissue lysates (35ug/lane).ATP6V1H (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-ATP6V1H antibody

Antibodies; Binding Proteins

References

Fuster, D.G., et al. Kidney Int. 73(10):1151-1158(2008). Stove, V., et al. J. Virol. 79(17):11422-11433(2005). Morel, N. Biol. Cell 95(7):453-457(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 47717104
• NCBI GeneID: 51606
• NCBI Accession #: NP_057025.2
• NCBI GenBank Nucleotide #: NM_015941.3
• UniProt Accession #: Q9UI12; Q6PK44; Q9H3E3; Q9Y300; B3KMR0
• Molecular Weight: 55,883 Da
• NCBI Official Full Name: V-type proton ATPase subunit H isoform 1
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
• NCBI Official Symbol: ATP6V1H
• NCBI Official Synonym Symbols: SFD; NBP1; VMA13; CGI-11; MSTP042; SFDbeta; SFDalpha
• NCBI Protein Information: V-type proton ATPase subunit H; V-ATPase subunit H; nef-binding protein 1; protein VMA13 homolog; vacuolar ATPase subunit H; V-ATPase 50/57 kDa subunits; vacuolar proton pump subunit H; vacuolar ATP synthase subunit H; vacuolar proton pump subunit SFD
• UniProt Protein Name: V-type proton ATPase subunit H
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V1H
• UniProt Synonym Gene Names: V-ATPase subunit H; NBP1
• UniProt Entry Name: VATH_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

ATP6V1H: Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes. Belongs to the V-ATPase H subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Energy Metabolism - oxidative phosphorylation; Hydrolase; EC 3.6.3.14

Chromosomal Location of Human Ortholog: 8q11.2

Cellular Component: lysosomal membrane; plasma membrane; vacuolar proton-transporting V-type ATPase, V1 domain; cytosol

Molecular Function: protein binding; ATPase activity; hydrogen ion transporting ATPase activity, rotational mechanism; enzyme regulator activity

Biological Process: interaction with host; vacuolar acidification; viral reproduction; cellular iron ion homeostasis; regulation of catalytic activity; metabolic process; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; endocytosis; transmembrane transport; regulation of defense response to virus by virus