Rabbit ATP6V1C1 Polyclonal Antibody | anti-ATP6V1C1 antibody

ATP6V1C1 Rabbit pAb

Cat. Num. AAA4759303

• Gene Names: ATP6V1C1; VATC; Vma5; ATP6C; ATP6D
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: ATP6V1C1; Polyclonal Antibody; ATP6V1C1 Rabbit pAb; ATPase H+ transporting V1 subunit C1; VATC; Vma5; ATP6C; ATP6D; anti-ATP6V1C1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: 0.6mg/ml (varies by lot)

• Applicable Applications for anti-ATP6V1C1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:100-1:200
• Immunogen: Fusion protein of human ATP6V1C1
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of ATP6V1C1 in Human cerebella tissue and Human cerebrum tissue lysates in order using ATP6V1C1 Rabbit pAb at dilution 1/500)

Immunohistochemistry (IHC)

(Immunohistochemistry of ATP6V1C1 in paraffin-embedded Human liver cancer tissue using ATP6V1C1 Rabbit pAb at dilution 1/100. Predicted cell location: Cytoplasm)

Related Product Information for anti-ATP6V1C1 antibody

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene is one of two genes that encode the V1 domain C subunit proteins and is found ubiquitously. This C subunit is analogous but not homologous to gamma subunit of F-ATPases. Previously, this gene was designated ATP6D.

NCBI and Uniprot Product Information

• NCBI GI #: 401329
• NCBI GeneID: 528
• UniProt Accession #: P21283
• Molecular Weight: 382
• NCBI Official Full Name: V-type proton ATPase subunit C 1
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
• NCBI Official Symbol: ATP6V1C1
• NCBI Official Synonym Symbols: VATC; Vma5; ATP6C; ATP6D
• NCBI Protein Information: V-type proton ATPase subunit C 1; V-ATPase C subunit; H+ -ATPase C subunit; V-ATPase subunit C 1; vacuolar proton pump C subunit; vacuolar ATP synthase subunit C; vacuolar proton pump subunit C 1; vacuolar proton pump, 42-kD subunit; H+-transporting ATPas
• UniProt Protein Name: V-type proton ATPase subunit C 1
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V1C1
• UniProt Synonym Gene Names: ATP6C; ATP6D; VATC; V-ATPase subunit C 1
• UniProt Entry Name: VATC1_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene is one of two genes that encode the V1 domain C subunit proteins and is found ubiquitously. This C subunit is analogous but not homologous to gamma subunit of F-ATPases. Previously, this gene was designated ATP6D. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V1C1: Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase C subunit family.

Protein type: Hydrolase; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation; Transporter; EC 3.6.3.14

Chromosomal Location of Human Ortholog: 8q22.3

Cellular Component: apical part of cell; lysosomal membrane; plasma membrane; cytoplasmic vesicle; proton-transporting two-sector ATPase complex; cytosol

Molecular Function: hydrogen-exporting ATPase activity, phosphorylative mechanism; protein binding; transporter activity; hydrogen ion transporting ATPase activity, rotational mechanism

Biological Process: interaction with host; proton transport; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport