Rabbit ATP5S Polyclonal Antibody | anti-ATP5S antibody

ATP5S Polyclonal Antibody

Cat. Num. AAA3005699

• Gene Names: DMAC2L; FB; ATPW; ATP5S; HSU79253
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ATP5S; Polyclonal Antibody; ATP5S Polyclonal Antibody; ATP synthase subunit s; mitochondrial; ATP synthase-coupling factor B; FB; Mitochondrial ATP synthase regulatory component factor B; ATPW; anti-ATP5S antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATP5S polyclonal antibody detects endogenous levels of ATP5S protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 215

• Applicable Applications for anti-ATP5S antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 73-118 of Human ATP5S.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ATP5S polyclonal antibody at 1:500 dilutionLane1:MCF-7 cell lysateLane2:Mouse lung tissue lysateLane3:Rat lung tissue lysate)

Related Product Information for anti-ATP5S antibody

ATP5S, also known as ATP synthase subunits, mitochondrial, ATP synthase-coupling factor B or ATP synthase, H+ transporting, mitochondrial F0 complex, subunits (factor B), is a 215 amino acid mitochondrial inner membrane protein that belongs to the ATP synthase subunits family. Involved in regulation of mitochondrial membrane ATP synthase, ATP5S is necessary for H+ conduction of ATP synthase. The ATP5S gene encodes subunits, also known as factor B, of the proton channel. This subunit is necessary for energy transduction in ATP synthase complexes. The ATP5S gene is conserved in chimpanzee, canine, bovine, mouse, rat, chicken, zebrafish and mosquito, and maps to human chromosome 14q21.3.

NCBI and Uniprot Product Information

• NCBI GI #: 51558774
• NCBI GeneID: 27109
• NCBI Accession #: NP_001003803.1
• NCBI GenBank Nucleotide #: NP_001003803.1
• UniProt Accession #: Q99766
• Molecular Weight: ~ 25kDa
• NCBI Official Full Name: ATP synthase subunit s, mitochondrial isoform a
• NCBI Official Synonym Full Names: distal membrane arm assembly complex 2 like
• NCBI Official Symbol: DMAC2L
• NCBI Official Synonym Symbols: FB; ATPW; ATP5S; HSU79253
• NCBI Protein Information: ATP synthase subunit s, mitochondrial
• UniProt Protein Name: ATP synthase subunit s, mitochondrial
• Protein Family: ATP synthase
• UniProt Gene Name: ATP5S
• UniProt Synonym Gene Names: ATPW; FB
• UniProt Entry Name: ATP5S_HUMAN

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. This gene encodes the subunit s, also known as factor B, of the proton channel. This subunit is necessary for the energy transduction activity of the ATP synthase complexes. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP5S: Involved in regulation of mitochondrial membrane ATP synthase. Necessary for H(+) conduction of ATP synthase. Facilitates energy-driven catalysis of ATP synthesis by blocking a proton leak through an alternative proton exit pathway. Belongs to the ATP synthase subunit s family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 14q21.3

Cellular Component: proton-transporting ATP synthase complex, coupling factor F(o); mitochondrial inner membrane

Molecular Function: metal ion binding; hydrogen ion transmembrane transporter activity

Biological Process: cellular metabolic process; proton transport; mitochondrial ATP synthesis coupled proton transport