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Western Blot (WB) (All lanes : Anti-ATP5I Antibody at 1:1000 dilutionLane 1: human liver lysateLane 2: human heart lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 8 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human ATP5I Polyclonal Antibody | anti-ATP5I antibody

ATP5I Antibody

Gene Names
ATP5I; ATP5K
Reactivity
Human
Applications
Western Blot
Synonyms
ATP5I; Polyclonal Antibody; ATP5I Antibody; Purified Rabbit Polyclonal Antibody (Pab); ATP synthase subunit e; mitochondrial; ATPase subunit e; ATP5K; anti-ATP5I antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Form/Format
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Sequence Length
69
Applicable Applications for anti-ATP5I antibody
Western Blot (WB)
Application Notes
WB ~~1:1000
Antigen Source
Human
Antigen Type
A Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-ATP5I Antibody at 1:1000 dilutionLane 1: human liver lysateLane 2: human heart lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 8 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-ATP5I Antibody at 1:1000 dilutionLane 1: human liver lysateLane 2: human heart lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 8 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-ATP5I antibody
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
Product Categories/Family for anti-ATP5I antibody
References
Fujiwara T.,et al.Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases. Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. Xu G.,et al.Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315(2009). Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011). Van Damme P.,et al.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
521
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
7933
NCBI Official Full Name
ATP synthase subunit e, mitochondrial
NCBI Official Synonym Full Names
ATP synthase, H+ transporting, mitochondrial Fo complex subunit E
NCBI Official Symbol
ATP5I
NCBI Official Synonym Symbols
ATP5K
NCBI Protein Information
ATP synthase subunit e, mitochondrial
UniProt Protein Name
ATP synthase subunit e, mitochondrial
Protein Family
UniProt Gene Name
ATP5I
UniProt Synonym Gene Names
ATP5K; ATPase subunit e
UniProt Entry Name
ATP5I_HUMAN

NCBI Description

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the e subunit of the Fo complex. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]

Uniprot Description

ATP5I: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane. Belongs to the ATPase e subunit family.

Protein type: Mitochondrial; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial proton-transporting ATP synthase complex

Molecular Function: ATPase activity; hydrogen ion transmembrane transporter activity; transmembrane transporter activity

Biological Process: cellular metabolic process; mitochondrial ATP synthesis coupled proton transport

Research Articles on ATP5I

Similar Products

Product Notes

The ATP5I atp5i (Catalog #AAA9216530) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP5I Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATP5I can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~1:1000. Researchers should empirically determine the suitability of the ATP5I atp5i for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP5I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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