Rabbit anti-Human ATP5B Polyclonal Antibody | anti-ATP5B antibody

ATP5B, ID (ATP Synthase Subunit beta, Mitochondrial, ATPSB, ATPMB) (FITC)

Cat. Num. AAA6464367

• Gene Names: ATP5B; ATPMB; ATPSB; HEL-S-271
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ATP5B; Polyclonal Antibody; ID (ATP Synthase Subunit beta; Mitochondrial; ATPSB; ATPMB) (FITC); anti-ATP5B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ATP5B.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ATP5B antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-1:50; FLISA: 1:1000; IHC: 1:50-1:100; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a portion within aa142-171 from the center region of human ATP5B conjugated to KLH.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATP5B Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate. ATP5B (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of ATP5B (arrow) using rabbit polyclonal ATP5B Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATP5B gene (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with ATP5B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ATP5B Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ATP5B antibody

Antibodies; Enzymes; Synthase

References

1. Neckelmann, N., et.al., Genomics 5(4), 829-843 (1989). 2. Ohta, S., et.al., J. Biol. Chem. 263(23), 11257-11262 (1988). 3. Wallace, D.C., et.al., Curr. Genet. 12(2), 81-90 (1987).

NCBI and Uniprot Product Information

• NCBI GI #: 32189394
• NCBI GeneID: 506
• NCBI Accession #: NP_001677
• NCBI GenBank Nucleotide #: NM_001686.3
• UniProt Accession #: P06576; Q14283; A8K4X0
• Molecular Weight: 56,560 Da
• NCBI Official Full Name: ATP synthase subunit beta, mitochondrial
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
• NCBI Official Symbol: ATP5B
• NCBI Official Synonym Symbols: ATPMB; ATPSB; HEL-S-271
• NCBI Protein Information: ATP synthase subunit beta, mitochondrial
• UniProt Protein Name: ATP synthase subunit beta, mitochondrial
• UniProt Gene Name: ATP5B
• UniProt Synonym Gene Names: ATPMB; ATPSB

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008]

Uniprot Description

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.