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Western Blot (WB)

Rabbit ATP5B Polyclonal Antibody | anti-ATP5B antibody

Anti-ATP5B Antibody

Reactivity
Human. Not yet tested in other species
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
ATP5B; Polyclonal Antibody; Anti-ATP5B Antibody; Anti-ATP6B Antibody; anti-ATP5B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human. Not yet tested in other species
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
Liquid
Concentration
100ug/100ul (varies by lot)
Applicable Applications for anti-ATP5B antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA titer: 1:1,000-1:10,000
Western blot analysis: 1:500-1:1,000
Immunohistochemistry: 1:50-500
Optimal dilutions/concentrations should be determined by the end user
Storage Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Immunogen
Rabbit polyclonal ATP5B antibody was raised against a 13 amino acid peptide at the C terminal residues of human ATP5B protein.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Western Blot (WB)

Immunohistochemistry (IHC) #2

Immunohistochemistry (IHC) #2
Related Product Information for anti-ATP5B antibody
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008]
References
(1) Chi,S.L., Wahl,M.L., Mowery,Y.M.,et al. Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase.Cancer Res. 67 (10), 4716-4724 (2007).

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Product Notes

The ATP5B (Catalog #AAA416102) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ATP5B Antibody reacts with Human. Not yet tested in other species and may cross-react with other species as described in the data sheet. AAA Biotech's ATP5B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA titer: 1:1,000-1:10,000 Western blot analysis: 1:500-1:1,000 Immunohistochemistry: 1:50-500 Optimal dilutions/concentrations should be determined by the end user. Researchers should empirically determine the suitability of the ATP5B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP5B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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