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Western Blot (WB) (Western blot analysis of ATP2B2 using COLO205 whole cell lysates)

Rabbit anti-Human, Rat ATP2B2 Polyclonal Antibody | anti-ATP2B2 antibody

ATP2B2 Antibody

Gene Names
ATP2B2; PMCA2; PMCA2a; PMCA2i
Reactivity
Human, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ATP2B2; Polyclonal Antibody; ATP2B2 Antibody; AT2B2_HUMAN; atp2b2; ATPase plasma membrane Ca2+ transporting 2; Plasma membrane calcium ATPase 2; Plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium pump isoform 2; Plasma membrane calcium transporting ATPase 2; Plasma membrane calcium-transporting ATPase 2; PMCA2; PMCA2a; PMCA2i; anti-ATP2B2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ATP2B2 antibody detects endogenous levels of total ATP2B2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1243
Applicable Applications for anti-ATP2B2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human ATP2B2
Subcellular Location
Cell Membrane
Tissue Specificity
Mainly expressed in brain cortex. Found in low levels in skeletal muscle, heart muscle, stomach, liver, kidney and lung. Isoforms containing segment B are found in brain cortex and at low levels in other tissues. Isoforms containing segments X and W are found at low levels in all tissues. Isoforms containing segment A and segment Z are found at low levels in skeletal muscle and heart muscle.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of ATP2B2 using COLO205 whole cell lysates)

Western Blot (WB) (Western blot analysis of ATP2B2 using COLO205 whole cell lysates)
Related Product Information for anti-ATP2B2 antibody
Function: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
Subunit Structure: Interacts with PDZD11.
Similarity: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
491
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 137 kDa
Predicted: 137 kDa
NCBI Official Full Name
plasma membrane calcium-transporting ATPase 2 isoform 1
NCBI Official Synonym Full Names
ATPase plasma membrane Ca2+ transporting 2
NCBI Official Symbol
ATP2B2
NCBI Official Synonym Symbols
PMCA2; PMCA2a; PMCA2i
NCBI Protein Information
plasma membrane calcium-transporting ATPase 2
UniProt Protein Name
Plasma membrane calcium-transporting ATPase 2
UniProt Gene Name
ATP2B2
UniProt Synonym Gene Names
PMCA2; PMCA2

NCBI Description

The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

Research Articles on ATP2B2

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Product Notes

The ATP2B2 atp2b2 (Catalog #AAA9610074) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP2B2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATP2B2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the ATP2B2 atp2b2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP2B2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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