Rabbit ATP1A2 Polyclonal Antibody | anti-ATP1A2 antibody

ATP1A2 (Sodium/Potassium Transporting ATPase subunit Alpha 2, FHM2, MHP2) (AP)

Cat. Num. AAA6407675

• Gene Names: ATP1A2; FHM2; MHP2
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: ATP1A2; Polyclonal Antibody; ATP1A2 (Sodium/Potassium Transporting ATPase subunit Alpha 2; FHM2; MHP2) (AP); anti-ATP1A2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes ATP1A2. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-ATP1A2 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human ATP1A2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of HEK-293 cell lines, using ATP1A2 antibody.)

Product Categories/Family for anti-ATP1A2 antibody

Antibodies; Enzymes; ATPase

NCBI and Uniprot Product Information

• NCBI GI #: 4502271
• NCBI GeneID: 477
• NCBI Accession #: NP_000693.1
• NCBI GenBank Nucleotide #: NM_000702.3
• UniProt Accession #: P50993; Q07059; Q5JW74; Q86UZ5; Q9UQ25; D3DVE4
• Molecular Weight: 112,265 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit alpha-2 proprotein
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, alpha 2 polypeptide
• NCBI Official Symbol: ATP1A2
• NCBI Official Synonym Symbols: FHM2; MHP2
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit alpha-2; sodium pump subunit alpha-2; Na(+)/K(+) ATPase alpha-2 subunit; Na+/K+ ATPase, alpha-B polypeptide; Na+/K+ ATPase, alpha-A(+) catalytic polypeptide; sodium-potassium ATPase catalytic subunit alpha-2; s
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit alpha-2
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: ATP1A2
• UniProt Synonym Gene Names: KIAA0778; Na(+)/K(+) ATPase alpha-2 subunit
• UniProt Entry Name: AT1A2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 2 subunit. Mutations in this gene result in familial basilar or hemiplegic migraines, and in a rare syndrome known as alternating hemiplegia of childhood. [provided by RefSeq, Oct 2008]

Uniprot Description

ATP1A2: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients. Defects in ATP1A2 are the cause of familial hemiplegic migraine type 2 (FHM2). FHM2 is a rare, severe, autosomal dominant subtype of migraine characterized by aura and some hemiparesis. Defects in ATP1A2 are a cause of alternating hemiplegia of childhood 1 (AHC1). AHC is typically distinguished from familial hemiplegic migraine by infantile onset of the symptoms and high prevalence of associated neurological deficits that become increasingly obvious with age. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

Protein type: Hydrolase; Membrane protein, multi-pass; Transporter, ion channel; Membrane protein, integral; Transporter; EC 3.6.3.9

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: T-tubule; cytoplasm; dendritic spine; plasma membrane; synapse; caveola; sodium:potassium-exchanging ATPase complex; endosome; vesicle

Molecular Function: metal ion binding; chaperone binding; sodium:potassium-exchanging ATPase activity; ATP binding

Biological Process: regulation of smooth muscle contraction; response to nicotine; cellular sodium ion homeostasis; negative regulation of heart contraction; metabolic process; adult locomotory behavior; regulation of striated muscle contraction; sodium ion transport; neurological control of breathing; cellular potassium ion homeostasis; negative regulation of striated muscle contraction; potassium ion import; regulation of vasoconstriction; reduction of cytosolic calcium ion concentration; regulation of blood pressure; ATP hydrolysis coupled proton transport; neurotransmitter uptake; visual learning; locomotion; transmembrane transport; regulation of the force of heart contraction; potassium ion transport; cardiac muscle contraction

Disease: Migraine, Familial Hemiplegic, 2; Alternating Hemiplegia Of Childhood 1