Rabbit anti-Rat ATP1A1 Polyclonal Antibody | anti-ATP1A1 antibody

Phospho-ATP1A1 (Ser23) Antibody

Cat. Num. AAA7132986

• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
• Synonyms: ATP1A1; Polyclonal Antibody; Phospho-ATP1A1 (Ser23) Antibody; Nkaa1b; anti-ATP1A1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
• Form/Format: Liquid; Supplied at 1.0mg/mL In Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol.

• Applicable Applications for anti-ATP1A1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Peptide sequence around phosphorylation site of Serine 23 (K-K-G(p)-K-K) derived from Rat Na,K-ATPase alpha1.
• Immunogen Species: Rat
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blot analysis of extracts from Rat brain tissue using Na,K-ATPase alpha1(Phospho-Ser23) Antibody.The lane on the left is treated with the antigen-specific peptide. )

Product Categories/Family for anti-ATP1A1 antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 21361181
• NCBI GeneID: 476
• NCBI Accession #: NP_000692.2
• NCBI GenBank Nucleotide #: NM_000701.7
• UniProt Accession #: P05023; Q16689; Q6LDM4; Q9UCN1; Q9UJ20; Q9UJ21; B2RBR6; B7Z3U6
• Molecular Weight: 112,896 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit alpha-1 isoform a
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• NCBI Official Symbol: ATP1A1
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit alpha-1; sodium pump 1; Na+/K+ ATPase 1; Na,K-ATPase alpha-1 subunit; sodium pump subunit alpha-1; Na+, K+ ATPase alpha subunit; Na(+)/K(+) ATPase alpha-1 subunit; Na, K-ATPase, alpha-A catalytic polypeptide; s
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit alpha-1
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: ATP1A1
• UniProt Synonym Gene Names: Na(+)/K(+) ATPase alpha-1 subunit
• UniProt Entry Name: AT1A1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

ATP1A1: the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na and K ions across the plasma membrane. This action creates the electrochemical gradient of Na and K, providing the energy for active transport of various nutrients. Two splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Transporter, ion channel; EC 3.6.3.9; Membrane protein, multi-pass; Hydrolase; Transporter

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: Golgi apparatus; protein complex; basolateral plasma membrane; endoplasmic reticulum; T-tubule; integral to membrane; caveola; membrane; apical plasma membrane; plasma membrane; melanosome; sodium:potassium-exchanging ATPase complex; sarcolemma; endosome; vesicle

Molecular Function: potassium ion binding; protein domain specific binding; protein binding; sodium ion binding; chaperone binding; phosphoric monoester hydrolase activity; sodium:potassium-exchanging ATPase activity; phosphoinositide 3-kinase binding; ankyrin binding; ADP binding; protein kinase binding; ATP binding

Biological Process: response to drug; cellular sodium ion homeostasis; negative regulation of heart contraction; membrane hyperpolarization; positive regulation of striated muscle contraction; regulation of sodium ion transport; cellular potassium ion homeostasis; negative regulation of glucocorticoid biosynthetic process; dephosphorylation; potassium ion import; regulation of blood pressure; positive regulation of heart contraction; transmembrane transport; regulation of the force of heart contraction; cardiac muscle contraction