Rabbit anti-Human ATP12A Polyclonal Antibody | anti-ATP12A antibody

ATP12A, ID (Potassium-transporting ATPase alpha Chain 2, Proton Pump, Non-gastric H(+)/K(+) ATPase Subunit alpha, ATP1AL1) (AP)

Cat. Num. AAA6464397

• Gene Names: ATP12A; HK; #945; ATP1AL1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATP12A; Polyclonal Antibody; ID (Potassium-transporting ATPase alpha Chain 2; Proton Pump; Non-gastric H(+)/K(+) ATPase Subunit alpha; ATP1AL1) (AP); anti-ATP12A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ATP12A.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ATP12A antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: IHC-P: 1:50-1:100; ELISA: 1:1000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa839-868 in the center region of human ATP12A.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATP12A Antibody (Center) in K562 cell line lysates (35ug/lane). ATP12A (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skin reacted with ATP12A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ATP12A Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ATP12A antibody

Antibodies; Enzymes; ATPase

References

1. Li, J., et.al., Kidney Int. 65(4), 1301-1310 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 83700225
• NCBI GeneID: 479
• NCBI Accession #: NP_001667.4
• NCBI GenBank Nucleotide #: NM_001676.5
• UniProt Accession #: P54707; Q13816; Q13817; Q16734; Q5W035; Q8N5U2
• Molecular Weight: 116,053 Da
• NCBI Official Full Name: potassium-transporting ATPase alpha chain 2 isoform 2
• NCBI Official Synonym Full Names: ATPase H+/K+ transporting non-gastric alpha2 subunit
• NCBI Official Symbol: ATP12A
• NCBI Official Synonym Symbols: HK; #945; ATP1AL1
• NCBI Protein Information: potassium-transporting ATPase alpha chain 2
• UniProt Protein Name: Potassium-transporting ATPase alpha chain 2
• Protein Family: Potassium-transporting ATPase
• UniProt Gene Name: ATP12A
• UniProt Synonym Gene Names: ATP1AL1

NCBI Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This gene encodes a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

ATP12A: Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for potassium absorption in various tissues. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.3.10; Energy Metabolism - oxidative phosphorylation; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 13q12.12|13q12.1-q12.3

Cellular Component: basolateral plasma membrane; hydrogen:potassium-exchanging ATPase complex; plasma membrane

Molecular Function: ATP binding; hydrogen:potassium-exchanging ATPase activity; metal ion binding; sodium:potassium-exchanging ATPase activity

Biological Process: establishment or maintenance of transmembrane electrochemical gradient; potassium ion homeostasis; potassium ion transport; regulation of pH; sodium ion transport