Rabbit anti-Rat ATP Binding Cassette Transporter G8 Polyclonal Antibody | anti-ABCG8 antibody

Polyclonal Antibody to ATP Binding Cassette Transporter G8 (ABCG8)

Cat. Num. AAA2033962

• Reactivity: Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: ATP Binding Cassette Transporter G8; Polyclonal Antibody; Polyclonal Antibody to ATP Binding Cassette Transporter G8 (ABCG8); ABC-G8; STSL; Gallbladder Disease 4; Sterolin 2; ATP-binding cassette sub-family G member 8; anti-ABCG8 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against ABCG8. It has been selected for its ability to recognize ABCG8 in immunohistochemical staining and western blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence Length: 673

• Applicable Applications for anti-ABCG8 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western blotting: 1:50-400; Immunohistochemistry in formalin fixed frozen section: 1:50-500; Enzyme-linked Immunosorbent Assay: 1:100-1:5000
• Fragment: ABCG8 (Met1~Gln271)
• Conjugate: No Conjugate
• Cross Reactivity: Rat
• Immunoglobulin Type: IgG
• Quality Control: Content: The quality control contains recombinant ABCG8 (Met1~Gln271) disposed in loading buffer.; Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.; 5uL per well when used in enhanced chemilumescent (ECL).; Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.; Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2072369
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded liver tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Kidney Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 26080338
• NCBI GeneID: 155192
• NCBI Accession #: NP_569098.2
• NCBI GenBank Nucleotide #: NM_130414.2
• UniProt Accession #: P58428; Q8CIQ5; Q923R7
• Molecular Weight: 75,820 Da
• NCBI Official Full Name: ATP-binding cassette sub-family G member 8
• NCBI Official Synonym Full Names: ATP binding cassette subfamily G member 8
• NCBI Official Symbol: Abcg8
• NCBI Protein Information: ATP-binding cassette sub-family G member 8
• UniProt Protein Name: ATP-binding cassette sub-family G member 8
• Protein Family: ABC transporter G family
• UniProt Gene Name: Abcg8

NCBI Description

ATP-binding cassette (ABC) transporter that regulates sterol absorption and excretion; mutation causes sitosterolemia [RGD, Feb 2006]

Uniprot Description

ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg2+- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG5 has ATPase activity.