Western Blot (WB)
(All lanes: Anti ATM at 0.5ug/mlWB: Recombinant Human ATM Protein 0.5ngPredicted bind size: 60KDObserved bind size: 60KD)
anti-Human ATM Polyclonal Antibody | anti-ATM antibody
Anti-ATM Antibody
Western Blot (WB)
(All lanes: Anti ATM at 0.5ug/mlWB: Recombinant Human ATM Protein 0.5ngPredicted bind size: 60KDObserved bind size: 60KD)
Western Blot (WB)
(All lanes: Anti ATM at 0.5ug/mlWB: Recombinant Human ATM Protein 0.5ngPredicted bind size: 60KDObserved bind size: 60KD)
Background: ATM(ataxia telangiectasia mutated), also known as TEL1 or TELO1, is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. The ATM gene is mapped to chromosome 11q22.3. ATM has an essential role in the reconstitutive capacity of hematopoietic stem cells but is not as important for the proliferation or differentiation of progenitors in a telomere-independent manner. ATM functions directly in the repair of chromosomal DNA double-stranded breaks by maintaining DNA ends in repair complexes generated during lymphocyte gene assembly.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]
Uniprot Description
ATM: an atypical kinase of the PIKK family. Regulates cell cycle checkpoints and DNA repair . May function as a tumor suppressor. Activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Involved in the activation of ABL1 and SAPK. Binds DNA ends and is part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. DNA damage promotes association with RAD17. LOF mutations associated with ataxia telangiectasia, causing progressive loss of motor control (ataxia), dilation of superficial blood vessels (telangiectasia), cancer and immune deficiency. Approximately 30% of cases develop tumors, mostly lymphomas and leukemias, due to defects in DNA damage repair. Somatic mutations seen in leukemias and lymphomas.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; EC 2.7.11.1; DNA repair, damage; Tumor suppressor; Kinase, protein; ATYPICAL group; PIKK family
Chromosomal Location of Human Ortholog: 11q22-q23
Cellular Component: chromosome, telomeric region; cytoplasmic membrane-bound vesicle; nuclear chromosome, telomeric region; nucleoplasm; nucleus; spindle
Molecular Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; DNA binding; DNA-dependent protein kinase activity; protein binding; protein complex binding; protein dimerization activity; protein N-terminus binding; protein serine/threonine kinase activity
Biological Process: brain development; cell cycle arrest; determination of adult life span; DNA damage induced protein phosphorylation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction resulting in induction of apoptosis; DNA double-strand break processing; DNA repair; DNA replication; DNA synthesis during DNA repair; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; heart development; histone phosphorylation; lipoprotein catabolic process; meiotic recombination; mitotic cell cycle spindle assembly checkpoint; negative regulation of B cell proliferation; neuron apoptosis; oocyte development; peptidyl-serine phosphorylation; positive regulation of apoptosis; positive regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of histone phosphorylation; positive regulation of neuron apoptosis; positive regulation of telomere maintenance via telomerase; pre-B cell allelic exclusion; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of autophagy; regulation of telomerase activity; response to DNA damage stimulus; response to hypoxia; response to ionizing radiation; signal transduction; somitogenesis; strand displacement; telomere clustering; telomere maintenance via telomerase; V(D)J recombination
Disease: Ataxia-telangiectasia; Breast Cancer
Research Articles on ATM
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Product Notes
The ATM atm (Catalog #AAA178035) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-ATM Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the ATM atm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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