Rabbit anti-Human ATM Polyclonal Antibody | anti-ATM antibody

Rabbit Polyclonal to Human ATM

Cat. Num. AAA241245

• Gene Names: ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: ATM; Polyclonal Antibody; Rabbit Polyclonal to Human ATM; Anti-ATM Antibody (C-Terminus) IHC-plus; A-T mutated; ATA; ATC; ATD; ATE; AT mutated; Ataxia telangiectasia; ATDC; Serine-protein kinase ATM; AT1; Ataxia telangiectasia mutated; TEL1; TELO1; Human ATM; anti-ATM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: peptide corresponding to 14 amino acids near the carboxy terminus of human ATM
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 3056

• Applicable Applications for anti-ATM antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (1 - 2 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: A peptide corresponding to 14 amino acids near the carboxy terminus of human ATM.
• Immunogen Type: Synthetic peptide
• Immunogen: ATM antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human ATM.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-ATM antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ATM antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of ATM in Human Lymph Node cells with ATM antibody at 10 ug/ml.)

Western Blot (WB)

(Western blot of ATM in Daudi whole cell lysate with ATM antibody at (A) 1 and (B) 2 ug/ml.)

Related Product Information for anti-ATM antibody

The ATM gene product is a 370 kD nuclear phosphoprotein with C-terminal homology to phosphatidylinositol 3-kinase. ATM is involved in the induction of a DNA damage control pathway. Mutations in the ATM gene cause neuronal degeneration, gonadal abnormalities, and immune deficiencies as part of the hereditary disease ataxia telangiectasia.

Product Categories/Family for anti-ATM antibody

Family: Protein Kinase; Subfamily: PI3/PI4

NCBI and Uniprot Product Information

• NCBI GI #: 71902540
• NCBI GeneID: 472
• NCBI Accession #: NP_000042.3
• NCBI GenBank Nucleotide #: NM_000051.3
• UniProt Accession #: Q13315; O15429; Q12758; Q16551; Q93007; Q9NP02; Q9UCX7; B2RNX5
• Molecular Weight: 350,687 Da
• NCBI Official Full Name: serine-protein kinase ATM
• NCBI Official Synonym Full Names: ATM serine/threonine kinase
• NCBI Official Symbol: ATM
• NCBI Official Synonym Symbols: AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
• NCBI Protein Information: serine-protein kinase ATM; AT mutated; A-T mutated; ataxia telangiectasia mutated; TEL1, telomere maintenance 1, homolog
• UniProt Protein Name: Serine-protein kinase ATM
• Protein Family: ATM interactor
• UniProt Gene Name: ATM
• UniProt Synonym Gene Names: A-T mutated
• UniProt Entry Name: ATM_HUMAN

NCBI Description

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Uniprot Description

ATM: an atypical kinase of the PIKK family. Regulates cell cycle checkpoints and DNA repair . May function as a tumor suppressor. Activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Involved in the activation of ABL1 and SAPK. Binds DNA ends and is part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. DNA damage promotes association with RAD17. LOF mutations associated with ataxia telangiectasia, causing progressive loss of motor control (ataxia), dilation of superficial blood vessels (telangiectasia), cancer and immune deficiency. Approximately 30% of cases develop tumors, mostly lymphomas and leukemias, due to defects in DNA damage repair. Somatic mutations seen in leukemias and lymphomas.

Protein type: Kinase, protein; Protein kinase, atypical; Tumor suppressor; DNA repair, damage; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; PIKK family

Chromosomal Location of Human Ortholog: 11q22-q23

Cellular Component: nucleoplasm; chromosome, telomeric region; cytoplasmic membrane-bound vesicle; spindle

Molecular Function: protein dimerization activity; protein serine/threonine kinase activity; protein binding; DNA binding; 1-phosphatidylinositol-3-kinase activity; protein complex binding; protein N-terminus binding; DNA-dependent protein kinase activity; histone serine kinase activity; ATP binding

Biological Process: lipoprotein catabolic process; DNA damage induced protein phosphorylation; positive regulation of apoptosis; protein amino acid autophosphorylation; heart development; pre-B cell allelic exclusion; negative regulation of B cell proliferation; signal transduction; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; protein amino acid phosphorylation; positive regulation of neuron apoptosis; double-strand break repair; mitotic cell cycle spindle assembly checkpoint; cell cycle arrest; telomere maintenance; somitogenesis; V(D)J recombination; DNA repair; double-strand break repair via homologous recombination; peptidyl-serine phosphorylation; neuron apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; meiotic recombination; response to hypoxia; brain development; response to ionizing radiation; positive regulation of DNA damage response, signal transduction by p53 class mediator; response to DNA damage stimulus; oocyte development

Disease: Breast Cancer; Ataxia-telangiectasia