Rabbit ATIC Polyclonal Antibody | anti-ATIC antibody

ATIC Antibody

Cat. Num. AAA9409532

• Gene Names: ATIC; PURH; AICAR; AICARFT; IMPCHASE; HEL-S-70p
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: ATIC; Polyclonal Antibody; ATIC Antibody; PURH; AICAR; AICARFT; IMPCHASE; anti-ATIC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total ATIC protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 592

• Applicable Applications for anti-ATIC antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human ATIC.
• Target Name: ATIC
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ATIC antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human esophagus using ATIC antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human thyroid cancer using ATIC antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using ATIC antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-ATIC antibody

This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria.

Product Categories/Family for anti-ATIC antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 20127454
• NCBI GeneID: 471
• NCBI Accession #: NP_004035.2
• NCBI GenBank Nucleotide #: NM_004044.6
• UniProt Accession #: P31939; Q13856; Q53S28; A8K202; E9PBU3
• Molecular Weight: 64,524 Da
• NCBI Official Full Name: bifunctional purine biosynthesis protein PURH
• NCBI Official Synonym Full Names: 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
• NCBI Official Symbol: ATIC
• NCBI Official Synonym Symbols: PURH; AICAR; AICARFT; IMPCHASE; HEL-S-70p
• NCBI Protein Information: bifunctional purine biosynthesis protein PURH
• UniProt Protein Name: Bifunctional purine biosynthesis protein PURH
• UniProt Gene Name: ATIC
• UniProt Synonym Gene Names: PURH
• UniProt Entry Name: PUR9_HUMAN

NCBI Description

This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria. [provided by RefSeq, Sep 2009]

Uniprot Description

ATIC: Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis. Defects in ATIC are the cause of AICAR transformylase/IMP cyclohydrolase deficiency (AICAR). A neurologically devastating inborn error of purine biosynthesis. Patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA- ribotide and its derivatives in erythrocytes and fibroblasts. AICAR causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness. Belongs to the PurH family.

Protein type: EC 2.1.2.3; Nucleotide Metabolism - purine; Hydrolase; Methyltransferase; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 3.5.4.10

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: membrane; mitochondrion; cytosol

Molecular Function: protein homodimerization activity; IMP cyclohydrolase activity; phosphoribosylaminoimidazolecarboxamide formyltransferase activity

Biological Process: purine ribonucleoside monophosphate biosynthetic process; tetrahydrofolate biosynthetic process; organ regeneration; response to inorganic substance; nucleobase, nucleoside and nucleotide metabolic process; dihydrofolate metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; nucleoside metabolic process; cerebellum development; cerebral cortex development; purine base metabolic process; 'de novo' IMP biosynthetic process

Disease: Aicar Transformylase/imp Cyclohydrolase Deficiency