Goat ATGL/Desnutrin Polyclonal Antibody | anti-PNPLA2 antibody

Goat anti-ATGL/Desnutrin Antibody

Cat. Num. AAA420222

• Gene Names: PNPLA2; ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: ATGL/Desnutrin; Polyclonal Antibody; Goat anti-ATGL/Desnutrin Antibody; ATGL; DESNUTRIN; PNPLA2; patatin-like phospholipase domain containing 2; 1110001C14Rik; DKFZp667M109; FP17548; PEDF-R; TTS-2.2; TTS2; adipose triglyceride lipase; pigment epithelium-derived factor; transport-secretion protein 2.2; triglyceride hydrolase; anti-PNPLA2 antibody

• Host: Goat
• Clonality: Polyclonal
• Specificity: Pease note this antibody was designed using the Mouse sequence NP_080078.1, which differs by 2 amino acids from the Human sequence NP_065109.1.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence: NIIEVSKEARKR
• Sequence Length: 504

• Applicable Applications for anti-PNPLA2 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1:32000.; Immunohistochemistry: In paraffin embedded Human Heart shows intense staining of distinct spots in the myocardial fibres in transverse section. Recommended concentration, 2-3 ug/ml.; Western Blot: Approx 60kDa band observed in Human Adipose lystates (calculated MW of 55.3kDa according to NP_065109.1). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Testis for this particular batch.
• Immunogen: Peptide with sequence C-NIIEVSKEARKR, from the internal region of the protein sequence according to NP_065109.1.
• Preparation and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Western Blot (WB)

((0.5ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

((2.5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 32698724
• NCBI GeneID: 57104
• NCBI Accession #: NP_065109.1
• NCBI GenBank Nucleotide #: NM_020376.3
• NCBI Official Full Name: patatin-like phospholipase domain-containing protein 2
• NCBI Official Synonym Full Names: patatin like phospholipase domain containing 2
• NCBI Official Symbol: PNPLA2
• NCBI Official Synonym Symbols: ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik
• NCBI Protein Information: patatin-like phospholipase domain-containing protein 2
• UniProt Protein Name: Patatin-like phospholipase domain-containing protein 2
• Protein Family: Patatin-like phospholipase domain-containing protein
• UniProt Gene Name: PNPLA2
• UniProt Synonym Gene Names: ATGL; TTS2
• UniProt Entry Name: PLPL2_HUMAN

NCBI Description

This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010]

Uniprot Description

PNPLA2: the rate-limiting lipolytic enzyme in mammals, flies, and yeast. Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Upregulated by exercise training in human skeletal muscle. Has acylglycerol transacylase activity. May act coordinately with HSL within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. Interacting with ABHD5 stimulates its triglyceride hydrolase activity. Despite a colocalization in lipid droplets, it probably does not interact with perilipin. Transcriptionally regulated by FOXO1A. Defects cause neutral lipid storage disease (NLSD), an autosomal recessive disorder characterized by the excessive accumulation of neutral lipids in multiple tissues. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Lipase; EC 3.1.1.3

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytosol; endoplasmic reticulum membrane; integral to membrane; lipid particle; plasma membrane

Molecular Function: acylglycerol O-acyltransferase activity; lipoprotein lipase activity; triacylglycerol lipase activity

Biological Process: sequestering of lipid; triacylglycerol catabolic process

Disease: Neutral Lipid Storage Disease With Myopathy