Western Blot (WB)
(Western Blot analysis of extracts of HT-29 cells, using ATG9A antibody.)
Rabbit ATG9A Polyclonal Antibody | anti-ATG9A antibody
ATG9A Polyclonal Antibody
Gene Names
ATG9A; mATG9; APG9L1; MGD3208
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
ATG9A; Polyclonal Antibody; ATG9A Polyclonal Antibody; Autophagy-related protein 9A; APG9-like 1; mATG9; APG9L1; anti-ATG9A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ATG9A polyclonal antibody detects endogenous levels of ATG9A protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
839
Applicable Applications for anti-ATG9A antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant protein of human ATG9A.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of HT-29 cells, using ATG9A antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of HT-29 cells, using ATG9A antibody.)
Related Product Information for anti-ATG9A antibody
Atg9a (autophagy-related protein 9A), also known as APG9-like 1, is a 839 amino acid, multi-pass membrane protein that localizes to the pre-autophagosomal structure (PAS). Isolation membranes are suggested to originate from the PAS, enwrapping cytoplasmic components to form a double membrane autophagosome, which then fuses with the vacuole. Ubiquitously expressed in human adult tissues, Atg9a cycles between the Golgi and endosomes and, with the autophagosome-specific marker LC3, plays a critical role in starvation-induced autophagosome formation. Three isoforms of Atg9a exist as a result of alternative splicing events.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 94kDa
NCBI Official Full Name
autophagy-related protein 9A
NCBI Official Synonym Full Names
autophagy related 9A
NCBI Official Symbol
ATG9A
NCBI Official Synonym Symbols
mATG9; APG9L1; MGD3208
NCBI Protein Information
autophagy-related protein 9A
UniProt Protein Name
Autophagy-related protein 9A
Protein Family
UniProt Gene Name
ATG9A
UniProt Synonym Gene Names
APG9L1
UniProt Entry Name
ATG9A_HUMAN
Uniprot Description
ATG9A: Plays a role in autophagy. Cycles between a juxta- nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and FAM48A. Interacts with FAM48A. Belongs to the ATG9 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle; Autophagy; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: endoplasmic reticulum membrane; recycling endosome; membrane; late endosome membrane; late endosome; integral to membrane; autophagic vacuole; cytoplasmic vesicle; trans-Golgi network; endosome
Molecular Function: protein binding
Biological Process: protein transport; protein localization in Golgi apparatus; mitochondrion degradation; innate immune response; autophagic vacuole formation
Research Articles on ATG9A
Similar Products
Product Notes
The ATG9A atg9a (Catalog #AAA3008993) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATG9A Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATG9A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ATG9A atg9a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG9A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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