Immunohistochemistry (IHC)
(Anti-ATG5-ATG12 Complex antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Rabbit ATG5-ATG12 Polyclonal Antibody | anti-ATG5-ATG12 antibody
Anti-ATG5-ATG12 Complex Antibody (aa262-275)
Reactivity
MousePredicted: Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
ATG5-ATG12; Polyclonal Antibody; Anti-ATG5-ATG12 Complex Antibody (aa262-275); Rabbit Polyclonal (IgG) to Mouse ATG5-ATG12 Complex; anti-ATG5-ATG12 antibody
Host
Rabbit
Reactivity
Mouse
Predicted: Human
Predicted: Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes an epitope within the C-Terminal (CT) region of the Atg5-Atg12 complex, which plays a key role in the regulation of autophagy, and is emerging as an important factor in the innate immune response to viruses (Hwang et al. 2012). The Atg5-Atg12 complex interacts with retinoic acida inducible gene 1 and the adaptor molecule IFN-beta promoter stimulator 1, through caspase recruitment domains, thereby acting as a negative regulator of the type I IFN production pathway, and as a contributor to RNA viral replication within the host cells (Takeshita et al 2008).
Purity/Purification
Affinity Purified
Form/Format
PBS, 0.09% sodium azide
Applicable Applications for anti-ATG5-ATG12 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 5 ug/ml
WB: 1-2 ug/ml
WB: 1-2 ug/ml
Immunogen
KLH conjugated synthetic peptide corresponding to amino acids 262-275 of mouse Atg5. The corresponding sequence differs by one amino acid in human.
Conjugation
Unconjugated
Epitope
aa262-275
Preparation and Storage
Store at 4 degree C or -20 degree C. Avoid repeated freezing and thawing.
Immunohistochemistry (IHC)
(Anti-ATG5-ATG12 Complex antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-ATG5-ATG12 Complex antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
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Product Notes
The ATG5-ATG12 (Catalog #AAA2400904) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ATG5-ATG12 Complex Antibody (aa262-275) reacts with Mouse Predicted: Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATG5-ATG12 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 5 ug/ml WB: 1-2 ug/ml. Researchers should empirically determine the suitability of the ATG5-ATG12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG5-ATG12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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