Rabbit anti-Human, Mouse ATG4D Polyclonal Antibody

ATG4D, ID (ATG4D, APG4D, AUTL4, Cysteine protease ATG4D, AUT-like 4 cysteine endopeptidase, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D)

Cat. Num. AAA641674

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: ATG4D; Polyclonal Antibody; ID (ATG4D; APG4D; AUTL4; Cysteine protease ATG4D; AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D); Anti -ATG4D; anti-ATG4D antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ATG4D antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: ATG4D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227~257 amino acids from the Center of human APG4D.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-APG4D Pab in mouse kidney tissue lysate. APG4D(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-hAPG4D-L235 Pab in 293 cell line lysates transiently transfected with the APG4D gene (2ug/lane). hAPG4D-L235(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(APG4D Antibody (L235) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the APG4D antibody detected the APG4D protein (arrow).)

Related Product Information for anti-ATG4D antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Product Categories/Family for anti-ATG4D antibody

Antibodies; Abs to Enzymes; Protease (Proteinase; Peptidase)