Rabbit anti-Human ATG13 Polyclonal Antibody | anti-ATG13 antibody

ATG13, Phosphorylated (S355) (ATG13, KIAA0652, Autophagy-related protein 13) (PE)

Cat. Num. AAA6275995

• Gene Names: ATG13; KIAA0652; PARATARG8
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATG13; Polyclonal Antibody; Phosphorylated (S355) (ATG13; KIAA0652; Autophagy-related protein 13) (PE); Autophagy-related protein 13; anti-ATG13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ATG13 antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ATG13 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S355 of human ATG13.
• Conjugate: PE
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of ATG13 Antibody (Phospho S355) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-ATG13 antibody

Autophagy factor required for autophagosome formation. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex.

Product Categories/Family for anti-ATG13 antibody

Antibodies; Autophagy

References

Ferreira, R.C., et al. Nat. Genet. 42(9):777-780(2010). Hosokawa, N., et al. Autophagy 5(7):973-979(2009). Mercer, C.A., et al. Autophagy 5(5):649-662(2009). Ganley, I.G., et al. J. Biol. Chem. 284(18):12297-12305(2009). Chan, E.Y., et al. Mol. Cell. Biol. 29(1):157-171(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 218082953
• NCBI GeneID: 9776
• NCBI Accession #: NP_001136145.1; NP_055556.2
• NCBI GenBank Nucleotide #: NM_001142673.2
• UniProt Accession #: O75143; Q53EN6; Q9BRL3; Q9H8B0; B4DFI4; D3DQQ1; D3DQQ2; E9PQZ8
• Molecular Weight: 60,155 Da
• NCBI Official Full Name: autophagy-related protein 13 isoform 1
• NCBI Official Synonym Full Names: autophagy related 13
• NCBI Official Symbol: ATG13
• NCBI Official Synonym Symbols: KIAA0652; PARATARG8
• NCBI Protein Information: autophagy-related protein 13
• UniProt Protein Name: Autophagy-related protein 13
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG13
• UniProt Synonym Gene Names: KIAA0652
• UniProt Entry Name: ATG13_HUMAN

Uniprot Description

ATG13: an autophagy factor required for autophagosome formation. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. Phosphorylated by ULK1 and ULK2. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Part of a complex consisting of ATG13, ULK1 and RB1CC1. Interacts with ATG101. Interacts with ULK1 and -2 via C-terminus. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Autophagy

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: mitochondrion; cytosol

Molecular Function: protein binding; protein kinase binding

Biological Process: autophagic vacuole formation