Rabbit anti-Human ATF4 Polyclonal Antibody | anti-ATF4 antibody

ATF4, Phosphorylated (S245) (Activating Transcription Factor 4, CREB2, TXREB, CREB-2, TAXREB67) (HRP)

Cat. Num. AAA6407492

• Gene Names: ATF4; CREB2; TXREB; CREB-2; TAXREB67
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: ATF4; Polyclonal Antibody; Phosphorylated (S245) (Activating Transcription Factor 4; CREB2; TXREB; CREB-2; TAXREB67) (HRP); anti-ATF4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ATF4, phosphorylated S245.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-ATF4 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding S245 of human ATF4.
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells using Phospho-ATF4-S245 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-ATF4-S245 antibody.)

Product Categories/Family for anti-ATF4 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 33469976
• NCBI GeneID: 468
• NCBI Accession #: NP_001666.2
• NCBI GenBank Nucleotide #: NM_001675.2
• UniProt Accession #: P18848; Q9UH31
• Molecular Weight: 40 kDa
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-4
• NCBI Official Synonym Full Names: activating transcription factor 4
• NCBI Official Symbol: ATF4
• NCBI Official Synonym Symbols: CREB2; TXREB; CREB-2; TAXREB67
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-4; DNA-binding protein TAXREB67; tax-responsive enhancer element B67; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; cyclic AMP-r
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-4
• Protein Family: Cyclic AMP-dependent transcription factor
• UniProt Gene Name: ATF4
• UniProt Synonym Gene Names: CREB2; TXREB; cAMP-dependent transcription factor ATF-4; CREB-2; cAMP-responsive element-binding protein 2; TaxREB67
• UniProt Entry Name: ATF4_HUMAN

NCBI Description

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]

Uniprot Description

ATF-4: a transcription factor that is a member of the leucine zipper family. Isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). Involved in cisplatin resistance. Other members of the family include AP-1 transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; neuron projection; cytoplasm; microtubule organizing center; nucleus

Molecular Function: protein C-terminus binding; protein binding; leucine zipper domain binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; amino acid metabolic process; unfolded protein response; positive regulation of apoptosis; negative regulation of potassium ion transport; positive regulation of transcription, DNA-dependent; gluconeogenesis; positive regulation of transcription from RNA polymerase I promoter; cellular response to glucose starvation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; gamma-aminobutyric acid signaling pathway