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Western Blot (WB) (Western Blot analysis of A549 cells using Acetyl-ATF-5 (K29) Polyclonal Antibody)

Rabbit ATF-5 Polyclonal Antibody | anti-ATF-5 antibody

ATF-5 (Acetyl-Lys29) Polyclonal Antibody

Gene Names
ATF5; ATFX; HMFN0395
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
ATF-5; Polyclonal Antibody; ATF-5 (Acetyl-Lys29) Polyclonal Antibody; ATF5; ATFX; Cyclic AMP-dependent transcription factor ATF-5; cAMP-dependent transcription factor ATF-5; Activating transcription factor 5; Transcription factor ATFx; anti-ATF-5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Acetyl-ATF-5 (K29) Polyclonal Antibody detects endogenous levels of ATF-5 protein only when acetylated at K29.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
282
Applicable Applications for anti-ATF-5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
Western blotting: 1:500 - 1:2000
ELISA: 1:20000
Not yet tested in other applications
Immunogen Type
peptide
Immunogen Description
Synthesized peptide derived from the human ATF-5 around the acetylation site of K29.
Modification
Acetyl-Lys29
Target Species
Human
Target Name
ATF-5
Preparation and Storage
Store at -20 degree C/1 year

Western Blot (WB)

(Western Blot analysis of A549 cells using Acetyl-ATF-5 (K29) Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of A549 cells using Acetyl-ATF-5 (K29) Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of A549 cells using Acetyl-ATF-5 (K29) Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of A549 cells using Acetyl-ATF-5 (K29) Polyclonal Antibody)
Product Categories/Family for anti-ATF-5 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,674 Da
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-5
NCBI Official Synonym Full Names
activating transcription factor 5
NCBI Official Symbol
ATF5
NCBI Official Synonym Symbols
ATFX; HMFN0395
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-5
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-5
UniProt Gene Name
ATF5
UniProt Synonym Gene Names
ATFX; cAMP-dependent transcription factor ATF-5
UniProt Entry Name
ATF5_HUMAN

Uniprot Description

ATF-5: Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Belongs to the bZIP family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm

Molecular Function: protein binding; heat shock protein binding; transcription corepressor activity

Biological Process: circadian rhythm; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; olfactory bulb interneuron development; multicellular organism growth; negative regulation of astrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; post-embryonic development; negative regulation of apoptosis

Research Articles on ATF-5

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Product Notes

The ATF-5 atf5 (Catalog #AAA9411212) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATF-5 (Acetyl-Lys29) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATF-5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Western blotting: 1:500 - 1:2000 ELISA: 1:20000 Not yet tested in other applications. Researchers should empirically determine the suitability of the ATF-5 atf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATF-5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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