Rabbit anti-Human ATAD3A Polyclonal Antibody | anti-ATAD3A antibody

ATAD3A Antibody

Cat. Num. AAA9629529

• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ATAD3A; Polyclonal Antibody; ATAD3A Antibody; AAA-ATPase TOB3; Atad3a; ATPase family AAA domain containing protein 3A; ATPase family AAA domain-containing protein 3; ATPase family; AAA domain containing 3A; FLJ10709; FLJ35514; Tob3; anti-ATAD3A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ATAD3A Antibody detects endogenous levels of total ATAD3A.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ATAD3A antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: The transmembrane domain and a C-terminal adjacent region contain all information necessary for mitochondrial targeting. Belongs to the AAA ATPase family.
• Subunit Structure: Can form homooligomers. Homodimer formation at the N-terminus may be regulated by ATP and is required for the interaction with the inner surface of the mitochondrial outer membrane and correct mitochondrial homeostasis. Interacts with components of the mitochondrial ribosome and with other proteins involved in mitochondrial RNA metabolism. May also interact with protein involved in lipid metabolism, including STARD9. May interact with FAM210A. Interacts with GADD45GIP1. Interacts with S100B in a Ca(+2)- and Zn(+2)-dependent manner; this interaction probably occurs in the cytosol prior to mitochondrial targeting. S100B could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. Interacts with HSP60/HSPD1. Forms heterooligomers with ATAD3B; this interaction may affect ATAD3A activity.
• Tissue Specificity: Overexpressed in lung adenocarcinomas (at protein level).
• Function: Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level. May play an important role in mitochondrial protein synthesis. May also participate in mitochondrial DNA replication. May bind to mitochondrial DNA D-loops and contribute to nucleoid stability. Required for enhanced channeling of cholesterol for hormone-dependent steroidogenesis.
• Note: In the mitochondrial inner membrane, enriched in sites with the potential to form contacts with the outer membrane (PubMed:20349121, PubMed:20154147). The N-terminal domain interacts with the inner surface of the mitochondrial outer membrane and the C-terminal domain localizes in a specific matrix compartment, where it is associated with nucleoids (PubMed:18063578).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-ATAD3A antibody

Mitochondrion inner membrane; Single-pass membrane protein; Mitochondrion matrix; Mitochondrion nucleoid; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 283436220
• NCBI GeneID: 55210
• NCBI Accession #: NP_060658.3
• NCBI GenBank Nucleotide #: NM_018188.3
• UniProt Accession #: Q9NVI7; Q5SV23; Q8N275; Q96A50; B3KPB3; D2K8Q1; G3V1I6
• Molecular Weight: 57,948 Da
• NCBI Official Full Name: ATPase family AAA domain-containing protein 3A isoform 1
• NCBI Official Synonym Full Names: ATPase family, AAA domain containing 3A
• NCBI Official Symbol: ATAD3A
• NCBI Protein Information: ATPase family AAA domain-containing protein 3A
• UniProt Protein Name: ATPase family AAA domain-containing protein 3A
• Protein Family: ATPase family AAA domain-containing protein
• UniProt Gene Name: ATAD3A
• UniProt Entry Name: ATD3A_HUMAN

NCBI Description

ATAD3A and ATAD3B (MIM 612317) are mitochondrial membrane proteins that contribute to the stabilization of large mitochondrial DNA (mtDNA)-protein complexes called nucleoids (He et al., 2007 [PubMed 17210950]).[supplied by OMIM, Sep 2008]

Uniprot Description

ATAD3A: Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level. May play an important in mitochondrial protein synthesis. May also participate in mitochondrial DNA replication. May bind to mitochondrial DNA D-loops and contribute to nucleoid stability. Required for enhanced channeling of cholesterol for hormone- dependent steroidogenesis. Belongs to the AAA ATPase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: ATP binding

Biological Process: cell growth; negative regulation of apoptosis