Rabbit anti-Mouse, Rat AT2 Receptor Polyclonal Antibody

Anti-AT2 Receptor (Extracellular)-ATTO-488 Antibody

Cat. Num. AAA4157623

• Reactivity: Mouse, Rat
• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Purity: Affinity Purified On Immobilized Antigen.
• Synonyms: AT2 Receptor; Polyclonal Antibody; Anti-AT2 Receptor (Extracellular)-ATTO-488 Antibody; Angiotensin II; anti-AT2 Receptor antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Won't recognize human AT2 Receptor.
• Purity/Purification: Affinity Purified On Immobilized Antigen.
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-AT2 Receptor antibody: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Immunogen: Peptide DNLNATGTNESAFNC corresponding to amino acid residues 21-35 of rat AT2 Receptor (Accession P35351).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Testing Data

Related Product Information for anti-AT2 Receptor antibody

Angiotensin II receptor type 2 or AT2 receptor is one of the receptors that bind the octapeptide hormone Angiotensin II (Ang II). Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects. Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. AT2 is also a GPCR, and is largely believed to have counter-regulatory roles to the ones exerted through the AT1 receptor. Hence, binding of Ang II to the AT2 receptor will result in growth arrest and apoptosis, vasodilatation and hypotension. Although the AT2 receptor is structurally a member of the GPCR superfamily, the signaling mechanisms elicited following AT2 receptor activation are not fully clarified. Recent evidence indicates that the AT2 receptor signals through the Gia2 and the Gia3 proteins and through the activation of phosphotyrosine phosphatases such as SHP-1. AT2 receptor distribution is more restricted than that of the AT1 receptor. The highest expression levels of AT2 have been found in the fetus, which is followed by a marked decrease shortly after birth. Nevertheless, significant AT2 receptor expression can be detected in the heart, kidney, vascular endothelial cells and brain, among others. The antibody can be used in immunocytochemistry, immunohistochemistry and Western blot analysis and will recognize AT2 receptor from rat and mouse samples. We are pleased to offer a new version of this antibody that is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. The antibody has been tested in immunocytochemical and immunohistochemical applications and is specially suited for experiments requiring simultaneous labeling of different markers