Western Blot (WB)
((0.5ug/ml) staining of Human Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Asporin/ASPN Polyclonal Antibody | anti-ASPN antibody
Goat anti-Asporin/ASPN Antibody
Western Blot: Approx 40kDa band observed in Human Skeletal Muscle and Human Liver lysates (calculated MW of 43.4kDa according to NP_060150.3). Recommended concentration: 0.5-1.5ug/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide.
Immunofluorescence: In ATDC5 cell line signal observed in cytoplasm and on the cell surface. Data from a prevous batch (goat) gratefully received from SNP Research Center, RIKEN, Japan.
ELISA: An anonymous customer found positive results in ELISA on Human recombinant protein.
Western Blot (WB)
((0.5ug/ml) staining of Human Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Human Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunofluorescence (IF)
(Staining of ATDC5 cells (Panels A and C) and DAPI (panels B and D). Data gratefuly received from Dr. Shiro Ikegawa, SNP Research Center, RIKEN, Japan.)
Immunofluorescence (IF)
(Staining of ATDC5 cells (Panels A and C) and DAPI (panels B and D). Data gratefuly received from Dr. Shiro Ikegawa, SNP Research Center, RIKEN, Japan.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cartilage extracellular protein that is member of the small leucine-rich proteoglycan family. The encoded protein may regulate chondrogenesis by inhibiting transforming growth factor-beta 1-induced gene expression in cartilage. This protein also binds collagen and calcium and may induce collagen mineralization. Polymorphisms in the aspartic acid repeat region of this gene are associated with a susceptibility to osteoarthritis, and also with intervertebral disc disease. Alternative splicing of this gene results in multiple transcript variants.[provided by RefSeq, Jul 2014]
Uniprot Description
ASPN: Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins. Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF- beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity. Genetic variations in ASPN are associated with susceptibility to osteoarthritis type 3 (OS3); also known as osteoarthritis of knee/hip. Osteoarthritis is a degenerative disease of the joints characterized by degradation of the hyaline articular cartilage and remodeling of the subchondral bone with sclerosis. Clinical symptoms include pain and joint stiffness often leading to significant disability and joint replacement. Susceptibility to osteoarthritis is conferred by a triplet repeat expansion polymorphism. ASPN allele having 14 aspartic acid repeats in the N-terminal region of the protein (D14), is overrepresented relative to the common allele having 13 aspartic acid repeats (D13). The frequency of the D14 allele increases with disease severity. The D14 allele is also overrepresented in individuals with hip osteoarthritis. Defects in ASPN are a cause of susceptibility to intervertebral disk disease (IDD). A common musculo- skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Susceptibility to intervertebral disk disease, particularly lumbar disk degeneration, is conferred by a triplet repeat expansion polymorphism. ASPN allele having 14 aspartic acid repeats in the N-terminal region of the protein (D14), is associated with the disorder in some populations (PubMed:18304494). Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Protein type: Secreted; Extracellular matrix; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 9q22
Cellular Component: cytoplasm; extracellular matrix; proteinaceous extracellular matrix
Molecular Function: calcium ion binding; collagen binding; protein kinase inhibitor activity
Biological Process: bone mineralization; cytokine and chemokine mediated signaling pathway; negative regulation of JAK-STAT cascade; negative regulation of protein kinase activity; negative regulation of transforming growth factor beta receptor signaling pathway
Disease: Intervertebral Disc Disease; Osteoarthritis Susceptibility 3
Research Articles on ASPN
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Product Notes
The ASPN aspn (Catalog #AAA420218) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Asporin/ASPN Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Asporin/ASPN can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 40kDa band observed in Human Skeletal Muscle and Human Liver lysates (calculated MW of 43.4kDa according to NP_060150.3). Recommended concentration: 0.5-1.5ug/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide. Immunofluorescence: In ATDC5 cell line signal observed in cytoplasm and on the cell surface. Data from a prevous batch (goat) gratefully received from SNP Research Center, RIKEN, Japan. ELISA: An anonymous customer found positive results in ELISA on Human recombinant protein. Researchers should empirically determine the suitability of the ASPN aspn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: IHENKVKKIQ KDT. It is sometimes possible for the material contained within the vial of "Asporin/ASPN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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