Rabbit anti-Human ASPN Polyclonal Antibody | anti-ASPN antibody

ASPN, ID (Asporin, Periodontal Ligament-associated Protein 1, PLAP-1, SLRR1C, PLAP1, UNQ215/PRO241) (MaxLight 490)

Cat. Num. AAA6464314

• Gene Names: ASPN; OS3; PLAP1; PLAP-1; SLRR1C
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ASPN; Polyclonal Antibody; ID (Asporin; Periodontal Ligament-associated Protein 1; PLAP-1; SLRR1C; PLAP1; UNQ215/PRO241) (MaxLight 490); anti-ASPN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ASPN.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-ASPN antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide between corresponding to a portion of amino acids within aa247-277 in the center region of human ASPN
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ASPN antibody (Center) in HL60 cell line lysates(35ug/lane). ASPN (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with ASPN Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of ASPN Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-ASPN antibody

Antibodies; Extracellular Matrix Proteins

References

1. Gruber, H.E., Arthritis Res. Ther. 11(2), R47 (2009). 2. Ikegawa, S., Curr. Med. Chem. 15(7), 724-728 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 301069322
• NCBI GeneID: 54829
• NCBI Accession #: NP_060150
• NCBI GenBank Nucleotide #: NM_017680
• UniProt Accession #: Q9BXN1
• Molecular Weight: 42kDa
• NCBI Official Full Name: asporin isoform 1 preproprotein
• NCBI Official Synonym Full Names: asporin
• NCBI Official Symbol: ASPN
• NCBI Official Synonym Symbols: OS3; PLAP1; PLAP-1; SLRR1C
• NCBI Protein Information: asporin
• UniProt Protein Name: Asporin
• Protein Family: Asporin
• UniProt Gene Name: ASPN
• UniProt Synonym Gene Names: PLAP1; SLRR1C; PLAP-1
• UniProt Entry Name: ASPN_HUMAN

NCBI Description

This gene encodes a cartilage extracellular protein that is member of the small leucine-rich proteoglycan family. The encoded protein may regulate chondrogenesis by inhibiting transforming growth factor-beta 1-induced gene expression in cartilage. This protein also binds collagen and calcium and may induce collagen mineralization. Polymorphisms in the aspartic acid repeat region of this gene are associated with a susceptibility to osteoarthritis, and also with intervertebral disc disease. Alternative splicing of this gene results in multiple transcript variants.[provided by RefSeq, Jul 2014]

Uniprot Description

ASPN: Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins. Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF- beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity. Genetic variations in ASPN are associated with susceptibility to osteoarthritis type 3 (OS3); also known as osteoarthritis of knee/hip. Osteoarthritis is a degenerative disease of the joints characterized by degradation of the hyaline articular cartilage and remodeling of the subchondral bone with sclerosis. Clinical symptoms include pain and joint stiffness often leading to significant disability and joint replacement. Susceptibility to osteoarthritis is conferred by a triplet repeat expansion polymorphism. ASPN allele having 14 aspartic acid repeats in the N-terminal region of the protein (D14), is overrepresented relative to the common allele having 13 aspartic acid repeats (D13). The frequency of the D14 allele increases with disease severity. The D14 allele is also overrepresented in individuals with hip osteoarthritis. Defects in ASPN are a cause of susceptibility to intervertebral disk disease (IDD). A common musculo- skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Susceptibility to intervertebral disk disease, particularly lumbar disk degeneration, is conferred by a triplet repeat expansion polymorphism. ASPN allele having 14 aspartic acid repeats in the N-terminal region of the protein (D14), is associated with the disorder in some populations (PubMed:18304494). Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Protein type: Secreted; Extracellular matrix; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: collagen binding; calcium ion binding

Biological Process: negative regulation of transforming growth factor beta receptor signaling pathway; bone mineralization

Disease: Intervertebral Disc Disease; Osteoarthritis Susceptibility 3