Western Blot (WB)
(Western Blot; Sample: Recombinant DARS2, Mouse.)
Rabbit anti-Mouse Aspartyl tRNA Synthetase 2 (DARS2) Polyclonal Antibody | anti-DARS2 antibody
Polyclonal Antibody to Aspartyl tRNA Synthetase 2, Mitochondrial (DARS2)
Gene Names
Dars2; 5830468K18Rik
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Aspartyl tRNA Synthetase 2 (DARS2); Polyclonal Antibody; Polyclonal Antibody to Aspartyl tRNA Synthetase 2; Mitochondrial (DARS2); AspRS; Aspartyl-tRNA synthetase; anti-DARS2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against DARS2. It has been selected for its ability to recognize DARS2 in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Ala390~Ser653
Applicable Applications for anti-DARS2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-2ug/mL; 1:500-2000
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
Immunogen Sequence
Ala390~Ser653
Cross Reactivity
Mouse
Conjugation
None
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Recombinant DARS2, Mouse.)
Western Blot (WB)
(Western Blot; Sample: Recombinant DARS2, Mouse.)
Product Categories/Family for anti-DARS2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,102 Da
NCBI Official Full Name
aspartate--tRNA ligase, mitochondrial
NCBI Official Synonym Full Names
aspartyl-tRNA synthetase 2 (mitochondrial)
NCBI Official Symbol
Dars2
NCBI Official Synonym Symbols
5830468K18Rik
NCBI Protein Information
aspartate--tRNA ligase, mitochondrial
UniProt Protein Name
Aspartate--tRNA ligase, mitochondrial
UniProt Gene Name
Dars2
UniProt Synonym Gene Names
AspRS
UniProt Entry Name
SYDM_MOUSE
Uniprot Description
DARS2: Defects in DARS2 are a cause of leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). LBSL is an autosomal recessive disease and is defined on the basis of a highly characteristic constellation of abnormalities observed by magnetic resonance imaging and spectroscopy. Affected individuals develop slowly progressive cerebellar ataxia, spasticity, and dorsal column dysfunction, sometimes with a mild cognitive deficit or decline. Belongs to the class-II aminoacyl-tRNA synthetase family.
Protein type: Mitochondrial; Translation; EC 6.1.1.12; Ligase
Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus
Molecular Function: aminoacyl-tRNA ligase activity; protein homodimerization activity; nucleic acid binding; nucleotide binding; aspartate-tRNA ligase activity; aspartate-tRNA(Asn) ligase activity; ATP binding; ligase activity
Biological Process: tRNA aminoacylation for protein translation; translation; tRNA aminoacylation
Research Articles on DARS2
Similar Products
Product Notes
The DARS2 dars2 (Catalog #AAA2112467) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Aspartyl tRNA Synthetase 2, Mitochondrial (DARS2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Aspartyl tRNA Synthetase 2 (DARS2) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-2ug/mL; 1:500-2000 IHC: 5-20ug/mL; 1:50-200 ICC: 5-20ug/mL; 1:50-200. Researchers should empirically determine the suitability of the DARS2 dars2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ala390~Ser 653. It is sometimes possible for the material contained within the vial of "Aspartyl tRNA Synthetase 2 (DARS2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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