Western Blot (WB)
(Western blotAll lanes: Aspartyl/asparaginyl beta-hydroxylase antibody at 2ug/mlLane 1:mouse stomach tissueLane 2:jurkat whole cell lysateLane 3:HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 86,35,26,24,34,84,30,22,29 kDaObserved band size: 86 kDa)
Rabbit anti-Human Aspartyl/asparaginyl beta-hydroxylase Polyclonal Antibody | anti-ASPH antibody
Rabbit anti-human Aspartyl/asparaginyl beta-hydroxylase polyclonal Antibody
IHC:1:20-1:200
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Western Blot (WB)
(Western blotAll lanes: Aspartyl/asparaginyl beta-hydroxylase antibody at 2ug/mlLane 1:mouse stomach tissueLane 2:jurkat whole cell lysateLane 3:HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 86,35,26,24,34,84,30,22,29 kDaObserved band size: 86 kDa)
Western Blot (WB)
(Western blotAll lanes: Aspartyl/asparaginyl beta-hydroxylase antibody at 2ug/mlLane 1:mouse stomach tissueLane 2:jurkat whole cell lysateLane 3:HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 86,35,26,24,34,84,30,22,29 kDaObserved band size: 86 kDa)
Western Blot (WB)
(Western blotAll lanes: ASPH antibody at 4 ug/mlLane 1: Mouse lung tissueLane 2: Mouse kidney tissueLane 3: Mouse brain tissueLane 4: Mouse thymus tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 86,35,26,24,34,84,30,22,29 kDaObserved band size: 85 kDa)
Western Blot (WB)
(Western blotAll lanes: ASPH antibody at 4 ug/mlLane 1: Mouse lung tissueLane 2: Mouse kidney tissueLane 3: Mouse brain tissueLane 4: Mouse thymus tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 86,35,26,24,34,84,30,22,29 kDaObserved band size: 85 kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney using MBS1489867 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney using MBS1489867 at dilution 1:100)
NCBI and Uniprot Product Information
NCBI Description
This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis. [provided by RefSeq, Sep 2009]
Uniprot Description
ASPH: Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins. Belongs to the aspartyl/asparaginyl beta-hydroxylase family. 9 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.11.16; Membrane protein, integral; Oxidoreductase
Chromosomal Location of Human Ortholog: 8q12.1
Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; integral to membrane; plasma membrane; sarcoplasmic reticulum lumen; sarcoplasmic reticulum membrane
Molecular Function: calcium ion binding; electron carrier activity; peptide-aspartate beta-dioxygenase activity; protein binding; structural constituent of muscle; structural molecule activity
Biological Process: activation of store-operated calcium channel activity; detection of calcium ion; limb morphogenesis; muscle contraction; negative regulation of cell proliferation; palate development; pattern specification process; peptidyl-aspartic acid hydroxylation; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; regulation of inositol-1,4,5-triphosphate receptor activity; regulation of protein stability; response to ATP; transmembrane transport
Research Articles on ASPH
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Product Notes
The ASPH asph (Catalog #AAA1489867) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Aspartyl/asparaginyl beta-hydroxylase polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Aspartyl/asparaginyl beta-hydroxylase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the ASPH asph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aspartyl/asparaginyl beta-hydroxylase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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