Western Blot (WB)
(Western blot analysis of ASM3A Antibody (C-term) in MDA-MB435 cell line lysates (35ug/lane). ASM3A (arrow) was detected using the purified Pab.)
Rabbit anti-Human ASM3A Polyclonal Antibody | anti-ASM3A antibody
ASM3A, CT (SMPDL3A, ASML3A, Acid sphingomyelinase-like phosphodiesterase 3a) (AP)
Gene Names
SMPDL3A; ASM3A; ASML3a; yR36GH4.1
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ASM3A; Polyclonal Antibody; CT (SMPDL3A; ASML3A; Acid sphingomyelinase-like phosphodiesterase 3a) (AP); SMPDL3A; Acid sphingomyelinase-like phosphodiesterase 3a; anti-ASM3A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-ASM3A antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ASM3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 330-360 from the C-terminal region of human ASM3A.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of ASM3A Antibody (C-term) in MDA-MB435 cell line lysates (35ug/lane). ASM3A (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ASM3A Antibody (C-term) in MDA-MB435 cell line lysates (35ug/lane). ASM3A (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(ASM3A Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ASM3A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ASM3A Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ASM3A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(ASM3A Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ASM3A Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-ASM3A antibody
References
Mungall, A.J., et al. Nature 425(6960):805-811(2003). Wright, K.O., et al. J. Urol. 168(6):2645-2649(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,285 Da
NCBI Official Full Name
acid sphingomyelinase-like phosphodiesterase 3a isoform a
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase acid like 3A
NCBI Official Symbol
SMPDL3A
NCBI Official Synonym Symbols
ASM3A; ASML3a; yR36GH4.1
NCBI Protein Information
acid sphingomyelinase-like phosphodiesterase 3a
UniProt Protein Name
Acid sphingomyelinase-like phosphodiesterase 3a
UniProt Gene Name
SMPDL3A
UniProt Synonym Gene Names
ASML3A; ASM-like phosphodiesterase 3a
UniProt Entry Name
ASM3A_HUMAN
Uniprot Description
SMPDL3A: Belongs to the acid sphingomyelinase family.
Protein type: EC 3.1.4.-; Secreted; Secreted, signal peptide; Phosphodiesterase
Chromosomal Location of Human Ortholog: 6q22.31
Cellular Component: extracellular space
Molecular Function: protein binding; sphingomyelin phosphodiesterase activity; hydrolase activity, acting on glycosyl bonds
Biological Process: sphingomyelin catabolic process
Research Articles on ASM3A
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Product Notes
The ASM3A smpdl3a (Catalog #AAA6275633) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ASM3A, CT (SMPDL3A, ASML3A, Acid sphingomyelinase-like phosphodiesterase 3a) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ASM3A can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ASM3A smpdl3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASM3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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