Rabbit ASIC3 Polyclonal Antibody | anti-ASIC3 antibody

ASIC3 Antibody

Cat. Num. AAA9629864

• Gene Names: ASIC3; ACCN3; TNaC1; DRASIC; SLNAC1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ASIC3; Polyclonal Antibody; ASIC3 Antibody; ACCN 3; Accn3; ACCN3_HUMAN; Acid sensing ion channel 3; Acid-sensing ion channel 3; Amiloride sensitive cation channel 3; Amiloride sensitive cation channel 3 testis; Amiloride-sensitive cation channel 3; ASIC 3; DRASIC; hASIC 3; hASIC3; hTNaC 1; hTNaC1; Modulatory subunit of ASIC 2a; Modulatory subunit of ASIC2a; Neuronal amiloride sensitive cation channel 3; Neuronal amiloride-sensitive cation channel 3; Proton gated cation channel subunit; SLNAC 1; SLNAC1; Testis sodium channel 1; TNaC 1; TNaC1; anti-ASIC3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ASIC3 Antibody detects endogenous levels of ASIC3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ASIC3 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human ASIC3.
• Conjugation: Unconjugated
• Similarity: The PDZ domain-binding motif is involved in interaction with LIN7A, GOPC and MAGI1. Belongs to the amiloride-sensitive sodium channel (TC 1. A. 6) family. ASIC3 subfamily.
• Post Translational Modifications: Phosphorylated by PKA. Phosphorylated by PKC. In vitro, PRKCABP/PICK-1 is necessary for PKC phosphorylation and activation of a ASIC3/ACCN3-ASIC2/ASIC2b channel, but does not activate a homomeric ASIC3 channel (By similarity).
• Subunit Structure: Homotrimer or heterotrimer with other ASIC proteins (By similarity). Interacts with STOM; this regulates channel activity. Interacts with DLG4 (By similarity). Interacts with LIN7B, MAGI1/BAIAP1, GOPC and ASIC2.
• Tissue Specificity: Expressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis.
• Function: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties.
• Note: Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-ASIC3 antibody

Cell membrane; Multi-pass membrane protein; Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 82582992
• NCBI GeneID: 9311
• UniProt Accession #: Q9UHC3; O60263; O75906; Q59FN9; Q9UER8; Q9UHC4; B2R9V0
• Molecular Weight: 44,999 Da
• NCBI Official Full Name: Acid-sensing ion channel 3
• NCBI Official Synonym Full Names: acid-sensing (proton-gated) ion channel 3
• NCBI Official Symbol: ASIC3
• NCBI Official Synonym Symbols: ACCN3; TNaC1; DRASIC; SLNAC1
• NCBI Protein Information: acid-sensing ion channel 3; testis sodium channel 1; modulatory subunit of ASIC2a; proton-gated cation channel subunit; amiloride-sensitive cation channel 3, testis; neuronal amiloride-sensitive cation channel 3
• UniProt Protein Name: Acid-sensing ion channel 3
• Protein Family: Acid-sensing ion channel
• UniProt Gene Name: ASIC3
• UniProt Synonym Gene Names: ACCN3; SLNAC1; TNAC1; ASIC3; hASIC3; hTNaC1
• UniProt Entry Name: ASIC3_HUMAN

NCBI Description

This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is an acid sensor and may play an important role in the detection of lasting pH changes. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 2 has been observed as proton-gated channels sensitive to gadolinium. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]

Uniprot Description

ASIC3: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties. Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC3 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ion channel; Membrane protein, integral; Channel, cation; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q35

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane

Molecular Function: amiloride-sensitive sodium channel activity; enterobactin transporter activity; cation channel activity; sodium channel activity; PDZ domain binding

Biological Process: detection of mechanical stimulus involved in sensory perception of pain; detection of chemical stimulus involved in sensory perception of pain; transport; response to heat; sensory perception; sensory perception of sour taste; enterobactin transport; response to acidity; detection of temperature stimulus involved in sensory perception of pain; signal transduction; transmembrane transport