Rabbit anti-Human ASGR1 Polyclonal Antibody | anti-ASGR1 antibody

ASGR1, NT (ASGR1, CLEC4H1, Asialoglycoprotein receptor 1, C-type lectin domain family 4 member H1, Hepatic lectin H1) (MaxLight 650)

Cat. Num. AAA6275630

• Gene Names: ASGR1; HL-1; ASGPR; ASGPR1; CLEC4H1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ASGR1; Polyclonal Antibody; NT (ASGR1; CLEC4H1; Asialoglycoprotein receptor 1; C-type lectin domain family 4 member H1; Hepatic lectin H1) (MaxLight 650); Hepatic lectin H1; anti-ASGR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ASGR1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-ASGR1 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human ASGR1. Swiss/UniProt Accession: P07306.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB231 cell line lysates (35ug/lane) usingMBS6007731.This demonstrates that MBS6007731 detected the ASGR1 protein (arrow).)

Related Product Information for anti-ASGR1 antibody

Partially deglycosylated plasma glycoproteins and immunoglobulin IgA2 allotypes are efficiently and specifically removed from circulation by a receptor-mediated process. The asialoglycoprotein receptor binds to desialylated (galactosyl-terminal) glycoproteins. It transports these glycoproteins via a series of membrane vesicles and tubules to an acidic-sorting organelle where the receptor and ligand dissociate. Then the receptor is recycled back to the cell surface and the ligand is transported to the lysosomes for degradation. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

Product Categories/Family for anti-ASGR1 antibody

Antibodies; Lectins

References

1. Yang, J., et al. Arch. Virol. 155(6):881-888 (2010). 2. Liu, J., et al. PLoS ONE 5 (9), E12934 (2010). 3. Sorensen, A.L., et al. Blood 114(8):1645-1654 (2009). 4. Oh, J.H., et al. Mamm. Genome 16(12):942-954 (2005). 5. Yik, J.H., et al. J. Biol. Chem. 277(43):40844-40852 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4502251
• NCBI GeneID: 432
• NCBI Accession #: NP_001662.1
• NCBI GenBank Nucleotide #: NM_001671.4
• UniProt Accession #: P07306; I3L1X1
• Molecular Weight: 29,144 Da
• NCBI Official Full Name: asialoglycoprotein receptor 1 isoform a
• NCBI Official Synonym Full Names: asialoglycoprotein receptor 1
• NCBI Official Symbol: ASGR1
• NCBI Official Synonym Symbols: HL-1; ASGPR; ASGPR1; CLEC4H1
• NCBI Protein Information: asialoglycoprotein receptor 1; hepatic lectin H1; C-type lectin domain family 4 member H1
• UniProt Protein Name: Asialoglycoprotein receptor 1
• Protein Family: Asialoglycoprotein receptor
• UniProt Gene Name: ASGR1
• UniProt Synonym Gene Names: CLEC4H1; ASGP-R 1; ASGPR 1; HL-1
• UniProt Entry Name: ASGR1_HUMAN

NCBI Description

This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the more abundant major subunit. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

ASGR1: Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: integral to plasma membrane; extracellular region

Molecular Function: protein binding; protein homodimerization activity; metal ion binding; asialoglycoprotein receptor activity; carbohydrate binding

Biological Process: receptor-mediated endocytosis; cellular response to extracellular stimulus