Western Blot (WB)
((0.1ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Ascl3 Polyclonal Antibody | anti-Ascl3 antibody
Goat anti-Ascl3 (mouse) Antibody
Gene Names
Ascl3; Sgn1; bHLHa42
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Ascl3; Polyclonal Antibody; Goat anti-Ascl3 (mouse) Antibody; achaete-scute complex homolog 3 (Drosophila); MGC129412; MGC129413; Sgn1; bHLHa42; ASH-3; ICRFP703B1614Q5.4; ICRFP703N2430Q5.4; achaete-scute complex homolog-like 3; achaete-scute homolog 3; bHLH transcriptional regulator Sgn-1; mASH-3; mASH3; Ascl3 antibody; achaete-scute complex homolog 3 (Drosophila) antibody; MGC129412 antibody; MGC129413 antibody; Sgn1 antibody; bHLHa42 antibody; ASH-3 antibody; ICRFP703B1614Q5.4 antibody; ICRFP703N2430Q5.4 antibody; achaete-scute homolog 3 antibody; bHLH transcriptional regulator Sgn-1 antibody; mASH-3 antibody; mASH3 antibody; Ascl3 (mouse); anti-Ascl3 antibody
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
HLPEDYLEKRLSK
Sequence Length
174
Applicable Applications for anti-Ascl3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Immunohistochemistry: Note: This product is proven unsuitable for IHC.
Western Blot: Approx 23kDa band observed in Mouse Liver lysates (calculated MW of 20.2kDa according to NP_064435.1). Recommended concentration: 0.1-0.3ug/ml.
Immunohistochemistry: Note: This product is proven unsuitable for IHC.
Western Blot: Approx 23kDa band observed in Mouse Liver lysates (calculated MW of 20.2kDa according to NP_064435.1). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-HLPEDYLEKRLSK, from the internal region of the protein sequence according to NP_064435.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.1ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,245 Da
NCBI Official Full Name
achaete-scute homolog 3
NCBI Official Synonym Full Names
achaete-scute family bHLH transcription factor 3
NCBI Official Symbol
Ascl3
NCBI Official Synonym Symbols
Sgn1; bHLHa42
NCBI Protein Information
achaete-scute homolog 3
UniProt Protein Name
Achaete-scute homolog 3
Protein Family
UniProt Gene Name
Ascl3
UniProt Synonym Gene Names
Mash3; Sgn1; ASH-3; mASH-3; mASH3
UniProt Entry Name
ASCL3_MOUSE
Uniprot Description
ASCL3: Transcriptional repressor. Inhibits myogenesis.
Protein type: Transcription factor; DNA-binding
Cellular Component: nucleus; transcription factor complex
Molecular Function: DNA binding; protein binding; protein dimerization activity
Biological Process: negative regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on Ascl3
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Product Notes
The Ascl3 ascl3 (Catalog #AAA422568) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Ascl3 (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Ascl3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Immunohistochemistry: Note: This product is proven unsuitable for IHC. Western Blot: Approx 23kDa band observed in Mouse Liver lysates (calculated MW of 20.2kDa according to NP_064435.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the Ascl3 ascl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HLPEDYLEKR LSK. It is sometimes possible for the material contained within the vial of "Ascl3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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