Goat Ascl1a Polyclonal Antibody | anti-ascl1a antibody

Ascl1a (Achaete-scute Homolog 1a, Zash-1a, Pituitary-absent Protein, ash, ash1a, asha, pia)

Cat. Num. AAA6013612

• Gene Names: ascl1a; ash; asha; zasha; zash1a; Zash-1a; id:ibd5085; im:7138587; si:dkey-264k15.2
• Applications: ELISA
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Ascl1a; Polyclonal Antibody; Ascl1a (Achaete-scute Homolog 1a; Zash-1a; Pituitary-absent Protein; ash; ash1a; asha; pia); Anti -Ascl1a (Achaete-scute Homolog 1a; anti-ascl1a antibody

• Host: Goat
• Clonality: Polyclonal
• Specificity: Recognizes Ascl1a. Species sequence homology: Zebrafish.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-ascl1a antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.; Dilution: ELISA: 1:8000
• Immunogen: Synthetic peptide corresponding to QCSNKSASKQAKRQR, from Ascl1a, at the internal region of the protein (NP_571294.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-ascl1a antibody

Transcriptional regulator. May mediate transcription activation by binding to the E box-containing promoter. Involved in neurogenesis. Required for the development of neurons in the epiphysis, acting partially redundantly with neurog1 and downstream of flh. Involved in maintaining rhombomere boundaries in the hindbrain, probably via up-regulation of delta expression. Also involved in pituitary development; required cell-autonomously in adenohypophyseal cells for endocrine differentiation and for survival of a subset of cells.

Product Categories/Family for anti-ascl1a antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 20373131
• NCBI GeneID: 30466
• NCBI Accession #: NP_571294.1
• NCBI GenBank Nucleotide #: NM_131219.1
• UniProt Accession #: Q90259
• Molecular Weight: 21,928 Da
• NCBI Official Full Name: achaete-scute homolog 1a
• NCBI Official Synonym Full Names: achaete-scute complex-like 1a (Drosophila)
• NCBI Official Symbol: ascl1a
• NCBI Official Synonym Symbols: ash; asha; zasha; zash1a; Zash-1a; id:ibd5085; im:7138587; si:dkey-264k15.2
• NCBI Protein Information: achaete-scute homolog 1a; pia; pituitary absent; pituitary-absent protein
• UniProt Protein Name: Achaete-scute homolog 1a
• Protein Family: Achaete-scute
• UniProt Gene Name: ascl1a
• UniProt Synonym Gene Names: ash; ash1a; asha; pia; Zash-1a
• UniProt Entry Name: ASL1A_DANRE

Uniprot Description

Function: Transcriptional regulator. May mediate transcription activation by binding to the E box-containing promoter

By similarity. Involved in neurogenesis. Required for the development of neurons in the epiphysis, acting partially redundantly with neurog1 and downstream of flh. Involved in maintaining rhombomere boundaries in the hindbrain, probably via up-regulation of delta expression. Also involved in pituitary development; required cell-autonomously in adenohypophyseal cells for endocrine differentiation and for survival of a subset of cells. Ref.4 Ref.5 Ref.6

Subunit structure: Efficient DNA binding requires dimerization with another bHLH protein

By similarity.

Subcellular location: Nucleus

Probable.

Tissue specificity: In the 24 hours embryo, expressed in the dorsal hindbrain in two bilaterally symmetrical lines of cells marking the boundary between the alar and basal plates, and ventrally in rhombomere 1 near the floor plate. Also expressed in embryonic adenohypophysis, telencephalon, diencephalon, epiphysis, ventral tegmentum, neural retina and spinal cord, in discrete regions distinct from those expressing ascl1b. In the 30 hours embryo, hindbrain expression is in stripes adjacent to rhombomere boundaries. Ref.1 Ref.4 Ref.5 Ref.6

Developmental stage: First detected at 12 hours post-fertilization (hpf), increasing over the next 24 hours, then decreasing between 48 and 72 hours. Not detected in adult. Ref.1

Disruption phenotype: In pituitary absent (pia) mutants, adenohypophyseal cells fail to express hormone genes and some become apoptotic. Ref.6

Sequence similarities: Contains 1 bHLH (basic helix-loop-helix) domain.