Rabbit ASAP2 Polyclonal Antibody | anti-ASAP2 antibody

ASAP2 Polyclonal Antibody

Cat. Num. AAA3007024

• Gene Names: ASAP2; PAP; PAG3; AMAP2; DDEF2; SHAG1; CENTB3; Pap-alpha
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ASAP2; Polyclonal Antibody; ASAP2 Polyclonal Antibody; Arf-GAP with SH3 domain; ANK repeat and PH domain-containing protein 2; Development and differentiation-enhancing factor 2; Paxillin-associated protein with ARF GAP activity 3; PAG3; Pyk2 C-terminus-associated protein; PAP; DDEF2; KIAA0400; anti-ASAP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ASAP2 polyclonal antibody detects endogenous levels of ASAP2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 961

• Applicable Applications for anti-ASAP2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human ASAP2
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ASAP2 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:sp2/0 whole cell lysate Lane3:H9C2 whole cell lysate)

Related Product Information for anti-ASAP2 antibody

DDEF2 (ADP ribosylation factor [ARF]- GTPase-activating protein [GAP] containing SH3, ANK repeats, and PH domain, PAP, PAG3, AMAP1, ZG14P, centaurin beta4) is a phospholipid-dependent ADP-ribosylation factor (ARF) GTPase-activating protein (ARF-GAP) that binds to protein-tyrosine kinases Src and focal adhesion kinase. ARF family GTP-binding proteins are regulators of membrane traffic and cytoskeletal organization. Modulation of ARF activity by DDEF2 is important for the regulation of focal adhesion assembly and/or organization by influencing the mechanisms responsible for the recruitment and organization of focal adhesion proteins paxillin and FAK. In spreading platelets, most endogenous DDEF2 is localized at peripheral focal adhesions. Pyk2 directly phosphorylates DDEF2 on tyrosine-308 and -782, and this event affects the phosphoinositide binding profile of DDEF2. DDEF2 is phosphorylated on tyrosine residues in cells expressing activated Src and tyrosine phosphorylation depends on a proline-rich class II Src SH3 binding site.

NCBI and Uniprot Product Information

• NCBI GI #: 206597522
• NCBI GeneID: 8853
• NCBI Accession #: NP_001128663
• NCBI GenBank Nucleotide #: NP_001128663.1
• UniProt Accession #: O43150
• Molecular Weight: ~ 112kDa
• NCBI Official Full Name: arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform b
• NCBI Official Synonym Full Names: ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
• NCBI Official Symbol: ASAP2
• NCBI Official Synonym Symbols: PAP; PAG3; AMAP2; DDEF2; SHAG1; CENTB3; Pap-alpha
• NCBI Protein Information: arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
• UniProt Protein Name: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
• Protein Family: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein
• UniProt Gene Name: ASAP2
• UniProt Synonym Gene Names: DDEF2; KIAA0400; PAG3; PAP
• UniProt Entry Name: ASAP2_HUMAN

NCBI Description

This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]