Rabbit anti-Mouse ASAP1 Polyclonal Antibody | anti-ASAP1 antibody

ASAP1, ID (ASAP1, DDEF1, KIAA1249, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1, 130kD phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein, ADP-ribosylation factor-directed GTPase-activating protein 1,

Cat. Num. AAA6275494

• Gene Names: ASAP1; PAP; PAG2; AMAP1; DDEF1; ZG14P; CENTB4
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ASAP1; Polyclonal Antibody; ID (ASAP1; DDEF1; KIAA1249; Arf-GAP with SH3 domain; ANK repeat and PH domain-containing protein 1; 130kD phosphatidylinositol 4; 5-bisphosphate-dependent ARF1 GTPase-activating protein; ADP-ribosylation factor-directed GTPase-activating protein 1; ; Development and differentiation-enhancing factor 1; PIP2-dependent ARF1 GAP; anti-ASAP1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ASAP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ASAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 359-388 amino acids from the Central region of human ASAP1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ASAP1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the ASAP1 antibody detected the ASAP1 protein (arrow).)

Related Product Information for anti-ASAP1 antibody

Possesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity).

Product Categories/Family for anti-ASAP1 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Muller, T., et al. Oncogene 29(16):2393-2403(2010). Nat. Genet. 41(7):824-828(2009). Jian, X., et al. J. Biol. Chem. 284(3):1652-1663(2009). Inoue, H., et al. Mol. Biol. Cell 19(10):4224-4237(2008). Lin, D., et al. Cancer Res. 68(11):4352-4359(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 46094081
• NCBI GeneID: 50807
• NCBI Accession #: NP_060952.2
• NCBI GenBank Nucleotide #: NM_018482.3
• UniProt Accession #: Q9ULH1; B2RNV3
• Molecular Weight: 125,897 Da
• NCBI Official Full Name: arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: ArfGAP with SH3 domain, ankyrin repeat and PH domain 1
• NCBI Official Symbol: ASAP1
• NCBI Official Synonym Symbols: PAP; PAG2; AMAP1; DDEF1; ZG14P; CENTB4
• NCBI Protein Information: arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1; DEF-1; centaurin, beta 4; PIP2-dependent ARF1 GAP; ARF GTPase-activating protein 1; development and differentiation-enhancing factor 1; ADP-ribosylation factor-directed GTPase-activat
• UniProt Protein Name: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
• Protein Family: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein
• UniProt Gene Name: ASAP1
• UniProt Synonym Gene Names: DDEF1; KIAA1249; ARF GTPase-activating protein 1; DEF-1
• UniProt Entry Name: ASAP1_HUMAN

NCBI Description

This gene encodes an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regulation of membrane trafficking and cytoskeleton remodeling. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]