Goat Arylsulfatase H Polyclonal Antibody | anti-ARSH antibody
Goat anti-Arylsulfatase H Antibody
Gene Names
ARSH; sulfatase
Reactivity
Expected from sequence similarity: Human, Dog
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Arylsulfatase H; Polyclonal Antibody; Goat anti-Arylsulfatase H Antibody; ARSH; arylsulfatase family; member H; sulfatase; arylsulfatase H; ARSH antibody; member H antibody; sulfatase antibody; arylsulfatase H antibody; anti-ARSH antibody
Host
Goat
Reactivity
Expected from sequence similarity: Human, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
LSDCQASKTPEL
Sequence Length
562
Applicable Applications for anti-ARSH antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Preliminary experiments gave an approx 45kDa band in Human Kidney lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.5kDa according to NP_001011719.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide.
Western Blot: Preliminary experiments gave an approx 45kDa band in Human Kidney lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.5kDa according to NP_001011719.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence LSDCQASKTPEL, from the internal region of the protein sequence according to NP_001011719.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,525 Da
NCBI Official Full Name
arylsulfatase H
NCBI Official Synonym Full Names
arylsulfatase family member H
NCBI Official Symbol
ARSH
NCBI Official Synonym Symbols
sulfatase
NCBI Protein Information
arylsulfatase H
UniProt Protein Name
Arylsulfatase H
Protein Family
UniProt Gene Name
ARSH
UniProt Synonym Gene Names
ASH
UniProt Entry Name
ARSH_HUMAN
NCBI Description
Sulfatases, such as ARSH, hydrolyze sulfate esters from sulfated steroids, carbohydrates, proteoglycans, and glycolipids. They are involved in hormone biosynthesis, modulation of cell signaling, and degradation of macromolecules (Sardiello et al., 2005 [PubMed 16174644]).[supplied by OMIM, Mar 2008]
Uniprot Description
ARSH: Belongs to the sulfatase family
Protein type: Membrane protein, integral; EC 3.1.6.-; Membrane protein, multi-pass; Hydrolase
Chromosomal Location of Human Ortholog: Xp22.33
Cellular Component: endoplasmic reticulum lumen; integral to membrane
Molecular Function: arylsulfatase activity; metal ion binding
Biological Process: glycosphingolipid metabolic process; post-translational protein modification
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Product Notes
The ARSH arsh (Catalog #AAA422078) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Arylsulfatase H Antibody reacts with Expected from sequence similarity: Human, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Arylsulfatase H can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Preliminary experiments gave an approx 45kDa band in Human Kidney lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.5kDa according to NP_001011719.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the ARSH arsh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LSDCQASKTP EL. It is sometimes possible for the material contained within the vial of "Arylsulfatase H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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