Western Blot (WB)
(Figure 1. Western blot analysis of ARSE/ASE/ARSL using anti-ARSE/ASE/ARSL antibody (MBS1753812).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hek293 whole cell lysatesLane 2: monkey Cos-7 whole cell lysatesLane 3: human HK-2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ARSE/ASE/ARSL antigen affinity purified polyclonal antibody (Catalog # MBS1753812) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for ARSE/ASE/ARSL at approximately 66KD. The expected band size for ARSE/ASE/ARSL is at 66KD. )
Rabbit ARSE/ASE/ARSL Polyclonal Antibody | anti-ARSL antibody
Anti-ARSE/ASE/ARSL Antibody
Western Blot (WB)
(Figure 1. Western blot analysis of ARSE/ASE/ARSL using anti-ARSE/ASE/ARSL antibody (MBS1753812).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hek293 whole cell lysatesLane 2: monkey Cos-7 whole cell lysatesLane 3: human HK-2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ARSE/ASE/ARSL antigen affinity purified polyclonal antibody (Catalog # MBS1753812) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for ARSE/ASE/ARSL at approximately 66KD. The expected band size for ARSE/ASE/ARSL is at 66KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of ARSE/ASE/ARSL using anti-ARSE/ASE/ARSL antibody (MBS1753812).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hek293 whole cell lysatesLane 2: monkey Cos-7 whole cell lysatesLane 3: human HK-2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ARSE/ASE/ARSL antigen affinity purified polyclonal antibody (Catalog # MBS1753812) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for ARSE/ASE/ARSL at approximately 66KD. The expected band size for ARSE/ASE/ARSL is at 66KD. )
2. Daniele, A., Parenti, G., d'Addio, M., Andria, G., Ballabio, A., Meroni, G. Biochemical characterization of arylsulfatase E and functional analysis of mutations found in patients with X-linked chondrodysplasia punctata. Am. J. Hum. Genet. 62: 562-572, 1998.
3. Franco, B., Meroni, G., Parenti, G., Levilliers, J., Bernard, L., Gebbia, M., Cox, L., Maroteaux, P., Sheffield, L., Rappold, G. A., Andria, G., Petit, C., Ballabio, A. A cluster of sulfatase genes on Xp22. 3: mutations in chondrodysplasia punctata (CDPX) and implications for warfarin embryopathy. Cell 81: 15-25, 1995.
NCBI and Uniprot Product Information
NCBI Description
Arylsulfatase E is a member of the sulfatase family. It is glycosylated postranslationally and localized to the golgi apparatus. Sulfatases are essential for the correct composition of bone and cartilage matrix. X-linked chondrodysplasia punctata, a disease characterized by abnormalities in cartilage and bone development, has been linked to mutations in this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ARSE: May be essential for the correct composition of cartilage and bone matrix during development. Has no activity toward steroid sulfates. Defects in ARSE are the cause of chondrodysplasia punctata X-linked recessive type 1 (CDPX1). CDP is a clinically and genetically heterogeneous disorder characterized by punctiform calcification of the bones. CDPX1 is a congenital defect of bone and cartilage development characterized by aberrant bone mineralization, severe underdevelopment of nasal cartilage, and distal phalangeal hypoplasia. This disease can also be induced by inhibition with the drug warfarin. Belongs to the sulfatase family.
Protein type: Hydrolase; EC 3.1.6.-
Chromosomal Location of Human Ortholog: Xp22.3
Cellular Component: Golgi stack; endoplasmic reticulum lumen
Molecular Function: arylsulfatase activity; metal ion binding
Biological Process: cellular protein metabolic process; sphingolipid metabolic process; glycosphingolipid metabolic process; post-translational protein modification; skeletal development
Disease: Chondrodysplasia Punctata 1, X-linked Recessive
Research Articles on ARSL
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Product Notes
The ARSL arse (Catalog #AAA1753812) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ARSE/ASE/ARSL Antibody reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's ARSE/ASE/ARSL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 0.25-0.5ug/ml|Human, Monkey|. Researchers should empirically determine the suitability of the ARSL arse for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARSE/ASE/ARSL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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