Rabbit ARSB / Arylsulfatase B Polyclonal Antibody | anti-ARSB antibody

Anti-ARSB / Arylsulfatase B Antibody (C-Terminus) IHC-plus

Cat. Num. AAA2400010

• Gene Names: ARSB; ASB; G4S; MPS6
• Reactivity: Mouse, Rat, Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Peptide Column Affinity Chromotography
• Synonyms: ARSB / Arylsulfatase B; Polyclonal Antibody; Anti-ARSB / Arylsulfatase B Antibody (C-Terminus) IHC-plus; Rabbit Polyclonal (IgG) to Human ARSB / Arylsulfatase B; Human ARSB / Arylsulfatase B; Arylsulfatase B; ASB; G4S; MPS6; anti-ARSB antibody

• Host: Rabbit
• Reactivity: Mouse, Rat, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ARSB antibody is human, mouse and rat reactive. At least two isoforms of ARSB are known to exist; this antibody only recognizes the longest isoform.
• Purity/Purification: Peptide Column Affinity Chromotography
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 533

• Applicable Applications for anti-ARSB antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC-P: 10 ug/ml; IF: 20 ug/ml; WB: 1 - 2 ug/ml; ; Optimal dilution to be determined by the researcher.
• Immunogen: ARSB / Arylsulfatase B antibody was raised against 16 amino acid peptide near the carboxy terminus of human ARSB. The immunogen is located within aa 460-510 of ARSB.
• Antigen Type: Synthetic Peptide
• Target: ARSB/Arylsulfate B
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Immunogen Species: ARSB/Arylsulfatase B antibody was raised against Human
• Usage Summary: ARSB antibody can be used for detection of ARSB by Western blot at 1 - 2 ug/ml. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 59 kDa Observed: 57kDa
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C for up to 3 months. Store at -20°C for up to 1 year. Avoid repeat freeze-thaw cycles

Immunohistochemistry (IHC)

(Anti-ARSB / Arylsulfatase B antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ARSB / Arylsulfatase B antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Immunofluorescence of ARSB in human lung tissue with ARSB antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot analysis of ARSB in mouse lung tissue lysate with ARSB antibody at (A) 1 and (B) 2 ug/ml.)

Related Product Information for anti-ARSB antibody

ARSB Antibody, Arylsulfatase B Antibody, ASB Antibody, G4S Antibody, MPS6 Antibody Description: Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 38569405
• NCBI GeneID: 411
• NCBI Accession #: NP_000037.2
• NCBI GenBank Nucleotide #: NM_000046.3
• UniProt Accession #: P15848; Q8N322; Q9UDI9; B2RC20
• Molecular Weight: Predicted MW: 59 kDa; Observed MW: 57kDa
• NCBI Official Full Name: arylsulfatase B isoform 1
• NCBI Official Synonym Full Names: arylsulfatase B
• NCBI Official Symbol: ARSB
• NCBI Official Synonym Symbols: ASB; G4S; MPS6
• NCBI Protein Information: arylsulfatase B
• UniProt Protein Name: Arylsulfatase B
• Protein Family: Arylsulfatase
• UniProt Gene Name: ARSB
• UniProt Synonym Gene Names: ASB; G4S
• UniProt Entry Name: ARSB_HUMAN

NCBI Description

Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ARSB: Defects in ARSB are the cause of mucopolysaccharidosis type 6 (MPS6); also known as Maroteaux-Lamy syndrome. MPS6 is an autosomal recessive lysosomal storage disease characterized by intracellular accumulation of dermatan sulfate. Clinical features can include abnormal growth, short stature, stiff joints, skeletal malformations, corneal clouding, hepatosplenomegaly, and cardiac abnormalities. A wide variation in clinical severity is observed. Arylsulfatase B activity is defective in multiple sulfatase deficiency (MSD). A clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Arylsulfatase B activity is impaired in multiple sulfatase deficiency due to mutations in SUMF1. SUMF1 mutations result in defective post-translational modification of ARSB at residue Cys- 91 that is not converted to 3-oxoalanine. Belongs to the sulfatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - glycosaminoglycan degradation; EC 3.1.6.12; Hydrolase

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: cell surface; endoplasmic reticulum lumen; Golgi apparatus; lysosomal lumen; lysosome; mitochondrion; rough endoplasmic reticulum

Molecular Function: arylsulfatase activity; metal ion binding; N-acetylgalactosamine-4-sulfatase activity

Biological Process: autophagy; carbohydrate metabolic process; cellular protein metabolic process; central nervous system development; chondroitin sulfate catabolic process; chondroitin sulfate metabolic process; glycosaminoglycan metabolic process; glycosphingolipid metabolic process; lysosomal transport; lysosome organization and biogenesis; post-translational protein modification; response to estrogen stimulus; response to methylmercury; response to nutrient; response to pH; sphingolipid metabolic process

Disease: Mucopolysaccharidosis Type Vi