Western Blot (WB)
(Figure 1. Western blot analysis of Arsb using anti-Arsb antibody (MBS1753448).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat Rh35 whole cell lysatesLane 2: rat C6 whole cell lysatesLane 3: mouse HEPA1-6 whole cell lysatesLane 4: mouse Neuro-2a whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Arsb antigen affinity purified polyclonal antibody (Catalog # MBS1753448) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Arsb at approximately 66-70KD. The expected band size for Arsb is at 60KD. )
Rabbit anti-Mouse, Rat Arsb Polyclonal Antibody | anti-Arsb antibody
Anti-Arsb Antibody
Gene Names
Arsb; As1; As-1; As-1r; As-1s; As-1t; As1-r; As1-s; As1-t; Asr-1; Ast-1; AI480648; 1110007C02Rik
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
Arsb; Polyclonal Antibody; Anti-Arsb Antibody; As1; As1-s; Arylsulfatase B; ASB; EC 3. 1. 6. 12; N-acetylgalactosamine-4-sulfatase; G4S; arylsulfatase B; anti-Arsb antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for Arsb detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Applicable Applications for anti-Arsb antibody
Western Blot (WB)
Application Notes
WB: 0.25-0.5ug/ml|Mouse, Rat|
Immunogen
A synthetic peptide corresponding to a sequence of mouse Arsb (KTLWLFDINQDPEERHD).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of Arsb using anti-Arsb antibody (MBS1753448).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat Rh35 whole cell lysatesLane 2: rat C6 whole cell lysatesLane 3: mouse HEPA1-6 whole cell lysatesLane 4: mouse Neuro-2a whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Arsb antigen affinity purified polyclonal antibody (Catalog # MBS1753448) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Arsb at approximately 66-70KD. The expected band size for Arsb is at 60KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Arsb using anti-Arsb antibody (MBS1753448).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat Rh35 whole cell lysatesLane 2: rat C6 whole cell lysatesLane 3: mouse HEPA1-6 whole cell lysatesLane 4: mouse Neuro-2a whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Arsb antigen affinity purified polyclonal antibody (Catalog # MBS1753448) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Arsb at approximately 66-70KD. The expected band size for Arsb is at 60KD. )
Related Product Information for anti-Arsb antibody
Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene
References
1. Crawley, A. C., Brooks, D. A., Muller, V. J., Petersen, B. A., Isaac, E. L., Bielicki, J., King, B. M., Boulter, C. D., Moore, A. J., Fazzalari, N. L., Anson, D. S., Byers, S., Hopwood, J. J. Enzyme replacement therapy in a feline model of Maroteaux-Lamy syndrome. J. Clin. Invest. 97: 1864-1873, 1996.
2. Crawley, A. C., Niedzielski, K. H., Isaac, E. L., Davey, R. C. A., Byers, S., Hopwood, J. J. Enzyme replacement therapy from birth in a feline model of mucopolysaccharidosis type VI. J. Clin. Invest. 99: 651-662, 1997.
3. Crawley, A. C., Yogalingam, G., Muller, V. J., Hopwood, J. J. Two mutations within a feline mucopolysaccharidosis type VI colony cause three different clinical phenotypes. J. Clin. Invest. 101: 109-119, 1998.
2. Crawley, A. C., Niedzielski, K. H., Isaac, E. L., Davey, R. C. A., Byers, S., Hopwood, J. J. Enzyme replacement therapy from birth in a feline model of mucopolysaccharidosis type VI. J. Clin. Invest. 99: 651-662, 1997.
3. Crawley, A. C., Yogalingam, G., Muller, V. J., Hopwood, J. J. Two mutations within a feline mucopolysaccharidosis type VI colony cause three different clinical phenotypes. J. Clin. Invest. 101: 109-119, 1998.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,647 Da
NCBI Official Full Name
arylsulfatase B
NCBI Official Synonym Full Names
arylsulfatase B
NCBI Official Symbol
Arsb
NCBI Official Synonym Symbols
As1; As-1; As-1r; As-1s; As-1t; As1-r; As1-s; As1-t; Asr-1; Ast-1; AI480648; 1110007C02Rik
NCBI Protein Information
arylsulfatase B; ASB; G4S; arylsulfatase B complex; arylsulfatase B regulation; arylsulfatase B structural; N-acetylgalactosamine-4-sulfatase; arylsulfatase B temporal regulation
UniProt Protein Name
Arylsulfatase B
Protein Family
UniProt Gene Name
Arsb
UniProt Synonym Gene Names
As1; As1-s; ASB; G4S
UniProt Entry Name
ARSB_MOUSE
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Product Notes
The Arsb arsb (Catalog #AAA1753448) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Arsb Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Arsb can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 0.25-0.5ug/ml|Mouse, Rat|. Researchers should empirically determine the suitability of the Arsb arsb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Arsb, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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