Goat anti-Human Arrestin 2, beta Polyclonal Antibody | anti-ARRB2 antibody

Arrestin 2, beta (ARRB2, ARB2, ARR2, DKFZp686L0365, HGNC:712)

Cat. Num. AAA620880

• Gene Names: ARRB2; ARB2; ARR2; BARR2; DKFZp686L0365
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Arrestin 2; beta; Polyclonal Antibody; beta (ARRB2; ARB2; ARR2; DKFZp686L0365; HGNC:712); Anti -Arrestin 2; anti-ARRB2 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human b-Arrestin 2.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile 40-50% glycerol, PBS.

• Applicable Applications for anti-ARRB2 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 3-10ug/ml with the appropriate secondary reagents to detect b-Arrestin 2 in paraffin-embedded sections of normal human cortex, hippocampus, and caudate putamen. Optimal dilutions should be determined by the individual laboratory.
• Immunogen: E. coli-derived full-length recombinant human b-Arrestin 2.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ARRB2 antibody

The Arrestin family consists of four members: Arrestin 1 (visual Arrestin), Arrestin 2 (b-Arrestin 1), Arrestin 3 (b-Arrestin 2), and Arrestin 4 (cone Arrestin). While visual and cone Arrestins are found almost exclusively in the retina, b-Arrestins 1 and 2 are ubiquitously expressed, and were initially described as negative regulators of G protein-coupled receptor (GPCR) signaling. More recently, b-Arrestins have been determined to serve as scaffolds for various signaling pathways, including the MAPK cascades activating ERK2, p38a, and JNK3. These b-Arrestin scaffolds tie together the appropriate kinases in series, forming a discreet signaling module that localizes components to specific subcellular environments and facilitates greater kinase activation.

Product Categories/Family for anti-ARRB2 antibody

Antibodies; Abs to Arrestin

NCBI and Uniprot Product Information

• NCBI GI #: 119610827
• NCBI GeneID: 409
• UniProt Accession #: P32121; Q0Z8D3; Q2PP19; Q6ICT3; Q8N7Y2; Q9UEQ6; B5B0C0; B7WPL3; D3DTK2; Q59EM5; Q68DZ5
• Molecular Weight: 46,106 Da
• NCBI Official Full Name: arrestin, beta 2, isoform CRA_c
• NCBI Official Synonym Full Names: arrestin, beta 2
• NCBI Official Symbol: ARRB2
• NCBI Official Synonym Symbols: ARB2; ARR2; BARR2; DKFZp686L0365
• NCBI Protein Information: beta-arrestin-2; arrestin 3; arrestin beta-2; OTTHUMP00000115951
• UniProt Protein Name: Beta-arrestin-2
• Protein Family: Beta-arrestin
• UniProt Gene Name: ARRB2
• UniProt Synonym Gene Names: ARB2; ARR2
• UniProt Entry Name: ARRB2_HUMAN

NCBI Description

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq]

Uniprot Description

ARRB2: a member of the arrestin/beta-arrestin protein family. These proteins participate in agonist-mediated desensitization of G-protein-coupled receptors. Acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Expressed at high levels in the CNS and peripheral blood leukocytes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: postsynaptic membrane; basolateral plasma membrane; endocytic vesicle; postsynaptic density; cytoplasm; dendritic spine; plasma membrane; coated pit; cytoplasmic vesicle; nucleus; cytosol

Molecular Function: angiotensin receptor binding; protein kinase B binding; protein domain specific binding; type 2A serotonin receptor binding; follicle stimulating hormone receptor binding; alpha-1B adrenergic receptor binding; alpha-1A adrenergic receptor binding; protein binding; enzyme binding; G-protein-coupled receptor binding; ubiquitin protein ligase binding; protein complex binding; mitogen-activated protein kinase binding; protein complex scaffold; type 1 angiotensin receptor binding; D1 dopamine receptor binding; receptor binding

Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; follicle-stimulating hormone signaling pathway; positive regulation of receptor internalization; protein ubiquitination; negative regulation of caspase activity; adult walking behavior; protein transport; transforming growth factor beta receptor signaling pathway; receptor internalization; negative regulation of interleukin-6 production; detection of temperature stimulus involved in sensory perception of pain; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; platelet activation; Notch signaling pathway; negative regulation of interleukin-12 production; negative regulation of natural killer cell mediated cytotoxicity; positive regulation of synaptic transmission, dopaminergic; negative regulation of tumor necrosis factor production; negative regulation of interleukin-1 beta production; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; G-protein coupled receptor internalization; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; negative regulation of toll-like receptor signaling pathway; negative regulation of protein ubiquitination; brain development; positive regulation of calcium ion transport; blood coagulation