Western Blot (WB)
(ARL8 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ARL8 antibody detected the ARL8 protein (arrow).)
Rabbit anti-Human ARL8 Polyclonal Antibody | anti-ARL8 antibody
ARL8, CT (ARL5B, ARL8, ADP-ribosylation factor-like protein 5B, ADP-ribosylation factor-like protein 8) (MaxLight 750)
Gene Names
ARL5B; ARL8
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
ARL8; Polyclonal Antibody; CT (ARL5B; ADP-ribosylation factor-like protein 5B; ADP-ribosylation factor-like protein 8) (MaxLight 750); ARL5B; ADP-ribosylation factor-like protein 8; anti-ARL8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-ARL8 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
ARL8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 129-161 amino acids from the C-terminal region of human ARL8.
Conjugate
MaxLight750
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(ARL8 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ARL8 antibody detected the ARL8 protein (arrow).)
Western Blot (WB)
(ARL8 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ARL8 antibody detected the ARL8 protein (arrow).)
Immunohistochemistry (IHC)
(ARL8 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARL8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ARL8 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARL8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(ARL8 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ARL8 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ARL8 antibody
ARL5B (ARL8) belongs to a family of proteins that are structurally similar to ADP-ribosylation factors (ARFs; see MIM 103180). ARLs and ARFs are part of the RAS superfamily of regulatory GTPases.
Product Categories/Family for anti-ARL8 antibody
References
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006). Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005). Sebald, E., et al. Gene 311, 147-151 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,375 Da
NCBI Official Full Name
ADP-ribosylation factor-like protein 5B
NCBI Official Synonym Full Names
ADP-ribosylation factor-like 5B
NCBI Official Symbol
ARL5B
NCBI Official Synonym Symbols
ARL8
NCBI Protein Information
ADP-ribosylation factor-like protein 5B; ADP-ribosylation factor-like 8; ADP-ribosylation factor-like protein 8; ADP-ribosylation-like factor 8
UniProt Protein Name
ADP-ribosylation factor-like protein 5B
Protein Family
UniProt Gene Name
ARL5B
UniProt Synonym Gene Names
ARL8
UniProt Entry Name
ARL5B_HUMAN
NCBI Description
ARL5B (ARL8) belongs to a family of proteins that are structurally similar to ADP-ribosylation factors (ARFs; see MIM 103180). ARLs and ARFs are part of the RAS superfamily of regulatory GTPases.[supplied by OMIM, Nov 2010]
Uniprot Description
ARL5B: Binds and exchanges GTP and GDP. Belongs to the small GTPase superfamily. Arf family.
Protein type: G protein, monomeric; G protein, monomeric, ARF
Chromosomal Location of Human Ortholog: 10p12.31
Cellular Component: intracellular
Molecular Function: GTP binding
Biological Process: small GTPase mediated signal transduction
Research Articles on ARL8
Similar Products
Product Notes
The ARL8 arl5b (Catalog #AAA6275202) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARL8, CT (ARL5B, ARL8, ADP-ribosylation factor-like protein 5B, ADP-ribosylation factor-like protein 8) (MaxLight 750) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARL8 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ARL8 arl5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARL8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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