Rabbit anti-Human ARL6 Polyclonal Antibody | anti-ARL6 antibody

ARL6 (ADP-Ribosylation Factor-like Protein 6, Bardet-Biedl Syndrome 3 Protein, ARL6, BBS3) (PE)

Cat. Num. AAA6454280

• Gene Names: ARL6; BBS3; RP55
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ARL6; Polyclonal Antibody; ARL6 (ADP-Ribosylation Factor-like Protein 6; Bardet-Biedl Syndrome 3 Protein; BBS3) (PE); anti-ARL6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ARL6. Species Crossreactivity: human
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ARL6 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 1:50-100; WB: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: KLH conjugated synthetic peptide corresponding to aa1-30 at the N-terminal region of human ARL6.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Categories/Family for anti-ARL6 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Pasqualato, S., et al., EMBO Rep. 3(11):1035-1041 (2002). Ingley, E., et al., FEBS Lett. 459(1):69-74 (1999). Jacobs, S., et al., FEBS Lett. 456(3):384-388 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 507834146
• NCBI GeneID: 84100
• NCBI Accession #: NP_001265222.1
• NCBI GenBank Nucleotide #: NM_001278293.1
• UniProt Accession #: Q9H0F7; A8KA93; D3DN31
• Molecular Weight: 21,960 Da
• NCBI Official Full Name: ADP-ribosylation factor-like protein 6
• NCBI Official Synonym Full Names: ADP-ribosylation factor-like 6
• NCBI Official Symbol: ARL6
• NCBI Official Synonym Symbols: BBS3; RP55
• NCBI Protein Information: ADP-ribosylation factor-like protein 6; Bardet-Biedl syndrome 3 protein
• UniProt Protein Name: ADP-ribosylation factor-like protein 6
• UniProt Gene Name: ARL6
• UniProt Synonym Gene Names: BBS3
• UniProt Entry Name: ARL6_HUMAN

NCBI Description

The protein encoded by this gene belongs to the ARF-like (ADP ribosylation factor-like) sub-family of the ARF family of GTP-binding proteins which are involved in regulation of intracellular traffic. Mutations in this gene are associated with Bardet-Biedl syndrome (BBS). Alternative splicing results in multiple transcript variants of this gene. A vision-specific transcript encoding a different protein has been described (PMID: 20333246). [provided by RefSeq, May 2013]

Uniprot Description

ARL6: Involved in membrane protein trafficking at the base of the ciliary organelle. Mediates recruitment onto plasma membrane of the BBSome complex which would constitute a coat complex required for sorting of specific membrane proteins to the primary cilia. May regulate cilia assembly and disassembly and subsequent ciliary signaling events such as the Wnt signaling cascade. Isoform 2 may be required for proper retinal function and organization. Defects in ARL6 are a cause of Bardet-Biedl syndrome type 3 (BBS3). Bardet-Biedl syndrome (BBS) is a genetically heterogeneous disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. Defects in ARL6 are the cause of retinitis pigmentosa type 55 (RP55). RP55 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the small GTPase superfamily. Arf family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric; G protein; G protein, monomeric, ARF

Chromosomal Location of Human Ortholog: 3q11.2

Cellular Component: membrane coat; membrane; cytoplasm; plasma membrane; axoneme; cytosol; cilium

Molecular Function: protein binding; GTP binding; metal ion binding; phospholipid binding

Biological Process: fat cell differentiation; protein polymerization; Wnt receptor signaling pathway; visual perception; small GTPase mediated signal transduction; organelle organization and biogenesis; cilium biogenesis; brain development; intermembrane transport; determination of left/right symmetry; protein targeting to membrane; melanosome transport; regulation of smoothened signaling pathway

Disease: Retinitis Pigmentosa 55; Bardet-biedl Syndrome 3; Bardet-biedl Syndrome 1; Retinitis Pigmentosa