Rabbit anti-Human ARHGEF1 Polyclonal Antibody | anti-ARHGEF1 antibody

ARHGEF1, NT (ARHGEF1, Rho guanine nucleotide exchange factor 1, 115kD guanine nucleotide exchange factor, Sub1.5) (Azide free) (HRP)

Cat. Num. AAA6274944

• Gene Names: ARHGEF1; LSC; GEF1; IMD62; LBCL2; SUB1.5; P115-RHOGEF
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ARHGEF1; Polyclonal Antibody; NT (ARHGEF1; Rho guanine nucleotide exchange factor 1; 115kD guanine nucleotide exchange factor; Sub1.5) (Azide free) (HRP); Sub1.5; anti-ARHGEF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 879

• Applicable Applications for anti-ARHGEF1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ARHGEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-192 amino acids from the N-terminal region of human ARHGEF1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ARHGEF1 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ARHGEF1 antibody detected the ARHGEF1 protein (arrow).)

Related Product Information for anti-ARHGEF1 antibody

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Product Categories/Family for anti-ARHGEF1 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Loirand, G., et al. Med Sci (Paris) 26 (6-7), 561-563 (2010): Takefuji, M., et al. J. Hum. Genet. 55(1):42-49(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Slattum, G., et al. J. Cell Biol. 186(5):693-702(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 39777604
• NCBI GeneID: 9138
• NCBI Accession #: NP_945328.1; NP_945353.1; NP_004697.2
• NCBI GenBank Nucleotide #: NP_945328.1
• UniProt Accession #: Q92888
• NCBI Official Full Name: rho guanine nucleotide exchange factor 1 isoform 3
• NCBI Official Synonym Full Names: Rho guanine nucleotide exchange factor 1
• NCBI Official Symbol: ARHGEF1
• NCBI Official Synonym Symbols: LSC; GEF1; IMD62; LBCL2; SUB1.5; P115-RHOGEF
• NCBI Protein Information: rho guanine nucleotide exchange factor 1
• UniProt Protein Name: Rho guanine nucleotide exchange factor 1
• Protein Family: Rho guanine nucleotide exchange factor
• UniProt Gene Name: ARHGEF1
• UniProt Synonym Gene Names: p115-RhoGEF; p115RhoGEF
• UniProt Entry Name: ARHG1_HUMAN

NCBI Description

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

Uniprot Description

ARHGEF1: Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2- induced RhoA activation. Interacts with RHOA, GNA12 and GNA13. Homooligomerizes through the coiled coil region. May interact with CCPG1. Interacts with CTNNAL1. Ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Rac/Rho; GEFs; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.13

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding; GTPase activator activity

Biological Process: cell proliferation; nerve growth factor receptor signaling pathway; negative regulation of axonogenesis; regulation of axonogenesis; Rho protein signal transduction